Iijima K, Shimoyama N, Shimoyama M, Mizuguchi T
Department of Anesthesiology, Chiba University School of Medicine, Japan.
J Clin Laser Med Surg. 1993 Aug;11(4):185-9. doi: 10.1089/clm.1993.11.185.
This study was designed to investigate the effect of the He-Ne laser (continuous wave, lambda = 632.8 nm, 8.5 mW in power) irradiation on human erythrocyte deformability. Blood samples were obtained from hematologically normal adult donors by venipuncture. Red cells were washed and adjusted to 30% Ht with 0.9% NaCl solution (pH 7.00). Red cell solution samples were assigned to three groups. Each sample was divided into seven 3-ml working aliquots. The aliquots in Group I were irradiated for 0 (control), 1, 3, 5, 10, 15, and 30 min within 2 hr after sampling. The aliquots in Group 2 and Group 3 were stored at 5 degrees C for 24 and 36 hr, respectively, and received similar irradiations after 12 hr (in both groups), 24 hr (in Group 2), and 36 hr (in Group 3) from sampling. Red cell deformability was measured by the Nuclepore filter filtration and presented as the filter filtration rate (FFR). The deformability shown as FFR was unchanged in Group 1 (fresh cell group) from the control value, but improved significantly in Groups 2 and 3 (damaged cell groups) after the irradiation. These results suggested that the irradiation of low-powered He-Ne lasers improved cytoskeletal protein activities in damaged erythrocytes.
本研究旨在探讨氦氖激光（连续波，波长=632.8nm，功率8.5mW）照射对人红细胞变形性的影响。通过静脉穿刺从血液学正常的成年供者获取血样。红细胞经洗涤后用0.9%氯化钠溶液（pH7.00）调整至血细胞比容为30%。红细胞溶液样本分为三组。每个样本分成7个3ml的工作等分试样。第一组的等分试样在采样后2小时内分别照射0（对照）、1、3、5、10、15和30分钟。第二组和第三组的等分试样分别在5℃下保存24小时和36小时，并在采样后12小时（两组）、24小时（第二组）和36小时（第三组）接受类似照射。通过核孔滤膜过滤法测量红细胞变形性，并以滤膜过滤率（FFR）表示。第一组（新鲜细胞组）中以FFR表示的变形性与对照值相比无变化，但照射后第二组和第三组（受损细胞组）的变形性显著改善。这些结果表明，低功率氦氖激光照射可改善受损红细胞的细胞骨架蛋白活性。
