McPherson J M
Recombinant Protein Development Department, Genzyme Corporation, Framingham, Massachusetts 01701.
Clin Mater. 1992;9(3-4):225-34. doi: 10.1016/0267-6605(92)90103-z.
Bovine demineralized bone powder and reconstituted bovine dermal collagen have been effectively utilized during the past several years to deliver a variety of growth factors in animal models of hard and soft tissue wound repair. Bone morphogenetic proteins have been delivered in a demineralized bone powder matrix to promote ectopic bone formation in the rat subcutaneous model with the objective of studying the process of endochondral bone formation and evaluating the utility of such factors in promoting repair of hard tissue defects. Reconstituted bovine dermal collagen gels and sponges, including composites of collagen and heparin, have been utilized to deliver growth factors such as platelet-derived growth factor (PDGF), transforming growth factor-beta (TGF-beta) and fibroblast growth factor (FGF) to study their effects in subcutaneous and incisional models of dermal wound repair. The results of these experimental animal studies have provided convincing evidence that the rheological properties, biocompatibility and resorbable nature of type I collagen make it an excellent delivery vehicle for evaluation of a variety of growth factors in human clinical studies of hard and soft tissue would repair.
在过去几年中，牛脱矿骨粉和重组牛真皮胶原蛋白已被有效地用于在硬组织和软组织伤口修复的动物模型中递送多种生长因子。骨形态发生蛋白已被递送至脱矿骨粉基质中，以促进大鼠皮下模型中的异位骨形成，目的是研究软骨内骨形成过程，并评估这些因子在促进硬组织缺损修复中的效用。重组牛真皮胶原蛋白凝胶和海绵，包括胶原蛋白与肝素的复合材料，已被用于递送生长因子，如血小板衍生生长因子（PDGF）、转化生长因子-β（TGF-β）和成纤维细胞生长因子（FGF），以研究它们在真皮伤口修复的皮下和切口模型中的作用。这些实验动物研究的结果提供了令人信服的证据，即I型胶原蛋白的流变学特性、生物相容性和可吸收性使其成为在硬组织和软组织伤口修复的人体临床研究中评估多种生长因子的理想递送载体。
