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绵羊弓形虫病,Sabin和Feldman染色试验中微量滴定系统的可靠性。

Toxoplasmosis in sheep, the reliability of a microtiter system in Sabin and Feldman's dye test.

作者信息

Waldeland H

出版信息

Acta Vet Scand. 1976;17(4):426-31. doi: 10.1186/BF03547897.

Abstract

Sera were tested in 2 series both by the conventional technique (dilution in tubes) and by the microtiter system. In 1 series of comparative examinations the tests were performed with the same batch of accessory factor serum and antigen preparation, and in another series the tests were performed at 2 different laboratories. There was a high correlation between the 2 methods, but the titres obtained by the microtiter system were about 1 to 2 twofold dilutions lower than those obtained by the conventional dilution technique. The 95 % confidence limits by the microtiter system were plus and minus 1 twofold dilution.

摘要

血清通过传统技术(试管稀释)和微量滴定系统进行了两个系列的检测。在一系列对比检测中,使用同一批次的辅助因子血清和抗原制剂进行检测,而在另一系列中,检测在两个不同实验室进行。两种方法之间存在高度相关性,但微量滴定系统获得的滴度比传统稀释技术获得的滴度低约1至2倍稀释度。微量滴定系统的95%置信限为正负1倍稀释度。

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