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Dyes as Microchemical Indicators of a New Immunity Phenomenon Affecting a Protozoon Parasite (Toxoplasma).作为一种影响原生动物寄生虫(弓形虫)的新免疫现象的微化学指示剂的染料。
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Recency of Toxoplasma gondii infections correlated with results obtained in dye test and enzyme-linked immunosorbent assay.刚地弓形虫感染的近期情况与染料试验和酶联免疫吸附测定的结果相关。
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An enzyme-linked immunosorbent assay for detection of IgM antibodies to Toxoplasma gondii: use for diagnosis of acute acquired toxoplasmosis.一种用于检测弓形虫IgM抗体的酶联免疫吸附测定法:用于急性获得性弓形虫病的诊断。
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Standardized quantitative enzyme-linked immunoassay for antibodies to Toxoplasma gondii.弓形虫抗体标准化定量酶联免疫测定法
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Use of enzyme-linked immunosorbent assays (ELISA) for detection of monoclonal antibodies: experience with antigens of Toxoplasma gondii.
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A microplate enzyme-immunoassay for toxoplasma antibody.一种用于检测弓形虫抗体的微孔板酶免疫测定法。
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[Immunoenzyme (ELISA) diagnosis of parasitic diseases using a modified micromethod. 2. Results for toxoplasmosis, amebiasis, trichinosis, hydatidosis and aspergillosis].[使用改良微量法进行寄生虫病的免疫酶(ELISA)诊断。2. 弓形虫病、阿米巴病、旋毛虫病、包虫病和曲霉病的结果]
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10
Immunoglobulin G and immunoglobulin M enzyme-linked immunosorbent assays and defined toxoplasmosis serological patterns.免疫球蛋白G和免疫球蛋白M酶联免疫吸附测定以及明确的弓形虫病血清学模式。
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酶联免疫吸附测定法(ELISA)与染色试验在检测山羊自然获得性弓形虫抗体中的比较。

Comparison between ELISA and dye test for detection of naturally acquired toxoplasma gondii antibodies in the goat.

作者信息

Berdal B P, Olsvik O, Almlid T, Larsen H J, Lorentzen-Styr A M

出版信息

Acta Vet Scand. 1983;24(1):65-73. doi: 10.1186/BF03546758.

DOI:10.1186/BF03546758
PMID:6869148
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8291278/
Abstract

Toxoplasma gondii IgG antibodies were measured in 212 goat sera, comparing the Sabin-Feldman dye-test and a three-layer sandwich enzyme-linked immunosorbent assay (ELISA). With 98 % concordance obtained between these 2 tests, the results are at the same paragon as for human sera. Accordingly, the ELISA sandwich procedure appears to be suitable for large-scale analysis of goat sera. The discordant 2 were ELISA positive and dye-test negative. One possible explanation of the divergent titres is given using an immunized goat model.

摘要

在212份山羊血清中检测了弓形虫IgG抗体,比较了Sabin-Feldman染色试验和三层夹心酶联免疫吸附测定(ELISA)。这两种检测方法的一致性达98%,结果与人类血清相当。因此,ELISA夹心检测法似乎适用于山羊血清的大规模分析。不一致的两份样本ELISA检测为阳性而染色试验检测为阴性。利用免疫山羊模型对不同滴度给出了一种可能的解释。