酶联免疫吸附测定法（ELISA）与染色试验在检测山羊自然获得性弓形虫抗体中的比较。
Comparison between ELISA and dye test for detection of naturally acquired toxoplasma gondii antibodies in the goat.
作者信息
Berdal B P, Olsvik O, Almlid T, Larsen H J, Lorentzen-Styr A M
出版信息
Acta Vet Scand. 1983;24(1):65-73. doi: 10.1186/BF03546758.
Abstract
Toxoplasma gondii IgG antibodies were measured in 212 goat sera, comparing the Sabin-Feldman dye-test and a three-layer sandwich enzyme-linked immunosorbent assay (ELISA). With 98 % concordance obtained between these 2 tests, the results are at the same paragon as for human sera. Accordingly, the ELISA sandwich procedure appears to be suitable for large-scale analysis of goat sera. The discordant 2 were ELISA positive and dye-test negative. One possible explanation of the divergent titres is given using an immunized goat model.
摘要
在212份山羊血清中检测了弓形虫IgG抗体，比较了Sabin-Feldman染色试验和三层夹心酶联免疫吸附测定（ELISA）。这两种检测方法的一致性达98%，结果与人类血清相当。因此，ELISA夹心检测法似乎适用于山羊血清的大规模分析。不一致的两份样本ELISA检测为阳性而染色试验检测为阴性。利用免疫山羊模型对不同滴度给出了一种可能的解释。
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