人类和大鼠组织中嘌呤磷酸核糖转移酶的发育变化。
Developmental changes in purine phosphoribosyltransferases in human and rat tissues.
作者信息
Adams A, Harkness R A
出版信息
Biochem J. 1976 Dec 15;160(3):565-76. doi: 10.1042/bj1600565.
Abstract
- The hypoxanthine/guanine and adenine phosphoribosyltransferase activities in a wide variety of human tissues were studied during their growth and development from foetal life onward. A wide range of activities develop after birth, with especially high values in the central nervous system and testes. 2. Postnatal development of hypoxanthine/guanine phosphoribosyltransferase was also defined in the rat. Although there were increases in the central nervous system and testes, there was also a rise in activity in the liver, which was less marked in man. 3. A sensitive radiochemical assay method, using dTTP to inhibit 5'-nucleotidase activity, suitable for tissue extracts, was developed. 4. No definite evidence of the existence of tissue-specific isoenzymes of hypoxanthine/guanine or adenine phosphoribosyltransferase was found. Hypoxanthine/guanine phosphoribosyltransferase in testes, however, had a significantly different thermal-denaturation rate constant. 5. The findings are discussed in an attempt to relate activity of hypoxanthine/guanine phosphoribosyltransferase to biological function. Growth as well as some developmental changes appear to be related to increase in the activity of this enzyme.
摘要
- 对多种人体组织中次黄嘌呤/鸟嘌呤和腺嘌呤磷酸核糖基转移酶的活性进行了研究，研究范围涵盖从胎儿期开始的生长发育过程。出生后，该酶活性呈现出广泛的变化范围，在中枢神经系统和睾丸中的活性尤其高。2. 还对大鼠体内次黄嘌呤/鸟嘌呤磷酸核糖基转移酶的出生后发育情况进行了界定。尽管中枢神经系统和睾丸中的酶活性有所增加，但肝脏中的活性也有所上升，不过在人类中这种上升不太明显。3. 开发了一种灵敏的放射化学测定方法，该方法使用dTTP抑制5'-核苷酸酶活性，适用于组织提取物。4. 未发现存在次黄嘌呤/鸟嘌呤或腺嘌呤磷酸核糖基转移酶的组织特异性同工酶的确切证据。然而，睾丸中的次黄嘌呤/鸟嘌呤磷酸核糖基转移酶具有显著不同的热变性速率常数。5. 对研究结果进行了讨论，试图将次黄嘌呤/鸟嘌呤磷酸核糖基转移酶的活性与生物学功能联系起来。生长以及一些发育变化似乎与该酶活性的增加有关。
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