美洲龙虾（Homarus americanus）蜕皮间期蛋白质和RNA合成过程中的热补偿

Thermal compensation in protein and RNA synthesis during the intermolt cycle of the American lobster, Homarus americanus.

McCarthy J F, Sastry A N, Tremblay G C

Biol Bull. 1976 Dec;151(3):538-47. doi: 10.2307/1540504.

Abstract

The in vitro rates of incorporation of precursors into protein and RNA and the concentration of RNA were measured in tissues of intermolt and premolt lobsters acclimated to 5 degrees C and 20 degrees C. Midgut gland, abdominal muscle and gill of intermolt lobsters respond to temperature acclimation by a compensatory translation of the rate-temperature (R-T) curves with respect to the rates of incorporation of 3H-leucine and 3H-uridine into the acid-insoluble fraction. Midgut gland and muscle of premolt animals exhibit either no compensation or inverse compensation; gill tissue exhibits a rotation of the R-T curve. 2. The existence of the complete de novo pathway of pyrimidine biosynthesis is demonstrated in the class Crustacea. NaH14 CO2 is incorporated into orotic acid and orotic-14 C-acid is incorporated into the acid-insoluble fraction. 3. Both the concentration of RNA and the rates of incorporation of precursors of both the salvage and de novo pyrimidine pathways are enhanced in the midgut gland of premolt lobsters, relative to intermolt tissue, under conditions of warm-acclimation.

摘要

测定了适应5摄氏度和20摄氏度的蜕皮间期和蜕皮前期龙虾组织中前体掺入蛋白质和RNA的体外速率以及RNA的浓度。蜕皮间期龙虾的中肠腺、腹肌和鳃通过对3H-亮氨酸和3H-尿苷掺入酸不溶性部分的速率进行速率-温度（R-T）曲线的补偿性平移来响应温度驯化。蜕皮前期动物的中肠腺和肌肉要么没有补偿，要么有反向补偿；鳃组织表现出R-T曲线的旋转。2. 在甲壳纲动物中证明了嘧啶生物合成完整的从头合成途径的存在。NaH14CO2掺入乳清酸，乳清酸-14C酸掺入酸不溶性部分。3. 在暖适应条件下，相对于蜕皮间期组织，蜕皮前期龙虾中肠腺中RNA的浓度以及补救和从头嘧啶途径前体的掺入速率均有所提高。