[Isolation of the most active DNA synthesizing cells of hematopoietic organs by fractionation on a density gradient of albumin].
作者信息
Moskaleva E Iu, Mazurik V K, Evseeva N K, Man'ko V M
出版信息
Biull Eksp Biol Med. 1976;82(11):1339-41.
PMID:1016715
Abstract
After separation of the bone marrow or the spleen suspension on the discontinuous albumin gradient cell fractions were obtained in which the activity of the DNA-dependent DNA polymerase, aspartate carbamoyl transferase, as well as the rate of the 14C-thymidine incorporation in the DNA was 2 to 3 times higher than in the original suspension. The most actively DNA-synthesizing cells were concentrated in the 5th-6th fractions when the osmolarity of 35% BSA was 370 mOsm, or in the 2nd-3rd fractions when the osmolarity of 35% BSA was 380 mOsm.
摘要
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