Goodman R M, Schmidt E C, Benjamin W B
Cell Differ. 1976 Dec;5(4):233-46. doi: 10.1016/0045-6039(76)90034-8.
Synthesis and transport of proteins to the cell nucleus during puff induction was studied in S. coprophila. Changes in grain distribution along chromosomes (L-methionine [35S] incorporation into protein) were correlated with puffs induced by ecdysterone in vitro; A pattern of specific labelling at the sites of incipient puffs was noted within 2 h after the addition of the hormone, i.e. grains on the chromosomes were in clusters, characteristic for this time point and not seen in the controls (where only non-specific labeling was noted 0-4 h). Characteristic chromosomal puffs appeared between 3-4 h after the addition of ecdysterone. It was concluded that during ecdysterone-induced puff formation in salivary gland chromosomes, proteins which had been previously synthesized were selectively transported from the cytoplasm to specific sites on the chromosomes.
在嗜粪蝇中研究了蛋白质合成及在胀泡诱导期间向细胞核的转运。沿染色体的银粒分布变化（L-甲硫氨酸[35S]掺入蛋白质）与体外蜕皮甾酮诱导的胀泡相关；添加激素后2小时内，在初始胀泡位点观察到特异性标记模式，即染色体上的银粒成簇，这是该时间点的特征，在对照中未见（对照在0 - 4小时仅观察到非特异性标记）。添加蜕皮甾酮后3 - 4小时出现特征性染色体胀泡。得出的结论是，在唾液腺染色体蜕皮甾酮诱导的胀泡形成过程中，先前合成的蛋白质从细胞质被选择性转运至染色体上特定位点。
