在毛角眼蕈蚊（双翅目：眼蕈蚊科）唾液腺染色体中基因活性的实验诱导
Experimental induction of gene activity in the salivary gland chromosomes of Trichosia pubescens (Diptera: Sciaridae).
作者信息
Amabis J M, Janczur C
出版信息
J Cell Biol. 1978 Jul;78(1):1-7. doi: 10.1083/jcb.78.1.1.
During the course of experiments with larvae of Trichosia pubescens, we have unexpectedly found that diethyl ether or chloroform anesthesia induces a large puff in a specific band in the polytene chromosomes of the salivary glands. This puff develops a few minutes after the treatment, attaining its maximum size after 60-100 min, and regresses completely 200 min after its activation. Through autoradiography, an intense incorporation of RNA precursors into that puff was observed. A few other smaller puffs are also induced by the treatment. The treatment with diethyl ether or chloroform does not induce puffing in the polytene cells of malpighian tubules and of midgut.
在用毛鬃尖眼蕈蚊幼虫进行实验的过程中，我们意外地发现，二乙醚或氯仿麻醉会在唾液腺多线染色体的特定条带中诱导出一个大的胀泡。这个胀泡在处理后几分钟出现，60 - 100分钟后达到最大尺寸，并在激活后200分钟完全消退。通过放射自显影，观察到RNA前体大量掺入到那个胀泡中。该处理还诱导出了其他一些较小的胀泡。二乙醚或氯仿处理不会在马氏管和中肠的多线细胞中诱导胀泡形成。
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