Mohn W W, Wilson A E, Bicho P, Moore E R
Department of Microbiology & Immunology, University of British Columbia, Vancouver, Canada.
Syst Appl Microbiol. 1999 Feb;22(1):68-78. doi: 10.1016/S0723-2020(99)80029-0.
Resin acids are tricyclic diterpenes which are synthesized by trees and are a major cause of toxicity of pulp mill effluents. Bacterial strains isolated from three different sources and which grow on resin acids were physiologically characterized. Eleven strains, representating distinct groups, were further characterized physiologically and phylogenetically. The isolates had distinct specificities for use, as growth substrates, of the different resin acids tested. The isolates also used fatty acids but were generally limited in use of other diverse substrates tested. According to their 16S rDNA sequences, the representative isolates are related to members of the genera, Sphingomonas, Zoogloea, Ralstonia, Burkholderia, Pseudomonas and Mycobacterium. Analysis of whole-cell fatty acid profiles generally supported those phylogenetic relationships. However, most of the isolated did not have high similarities to reference strains in the Microbial Identification System database of fatty acid profiles or in the Biolog database of substrate oxidation patterns. Described species of Sphingomonas, Zoolgoea, Burkholderia Pseudomonas, most closely related to the isolates we characterized, failed to grow on, or degrade, resin acids. We propose recognition of Zoogloea resiniphila sp. nov., Pseudomonas vancouverensis sp. nov., P. abietaniphila sp. nov. and P. multiresinivorans sp. nov.
树脂酸是树木合成的三环二萜类化合物，是纸浆厂废水毒性的主要来源。从三个不同来源分离出的、能在树脂酸上生长的细菌菌株进行了生理特性鉴定。代表不同类群的11株菌株进一步进行了生理和系统发育特征分析。这些分离株对所测试的不同树脂酸作为生长底物具有不同的特异性。这些分离株也利用脂肪酸，但对所测试的其他多种底物的利用通常有限。根据它们的16S rDNA序列，代表性分离株与鞘氨醇单胞菌属、动胶菌属、罗尔斯通氏菌属、伯克霍尔德菌属、假单胞菌属和分枝杆菌属的成员相关。全细胞脂肪酸谱分析总体上支持了这些系统发育关系。然而，大多数分离株在脂肪酸谱的微生物鉴定系统数据库或底物氧化模式的Biolog数据库中与参考菌株的相似度不高。与我们鉴定的分离株关系最密切的鞘氨醇单胞菌属、动胶菌属、伯克霍尔德菌属、假单胞菌属的已描述物种不能在树脂酸上生长或降解树脂酸。我们提议承认动胶菌属嗜树脂新种、温哥华假单胞菌新种、冷杉假单胞菌新种和多树脂降解假单胞菌新种。
