Belova Svetlana E, Pankratov Timofei A, Detkova Ekaterina N, Kaparullina Elena N, Dedysh Svetlana N
S.N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-letya Octyabrya 7/2, Moscow 117312, Russia.
Int J Syst Evol Microbiol. 2009 Sep;59(Pt 9):2283-90. doi: 10.1099/ijs.0.009209-0. Epub 2009 Jul 20.
Three obligately aerobic, heterotrophic bacteria, designated strains WM1T, TPB606T and TPB621, were isolated from acidic Sphagnum-dominated tundra and Siberian wetlands in Russia. Cells of these isolates were Gram-negative, non-motile coccobacilli that occurred singly, in pairs or in chains, and were covered by large capsules. The novel strains were moderately acidophilic and psychrotolerant organisms capable of growth at pH 3.0-7.6 and 2-30 degrees C. Cells contained numerous intracellular poly-beta-hydroxybutyrate granules (3-4 per cell). The major cellular fatty acid was cyclo C19:0omega8c and the predominant quinone was Q-10. Strains TPB606T and TPB621, isolated from Siberian wetland, possessed almost identical 16S rRNA gene sequences and shared 97.2% sequence similarity with tundra strain WM1T. The three strains were shown to belong to the Alphaproteobacteria, but were related only distantly to the type strains of acidophilic bacteria Acidisphaera rubrifaciens (93.4-94.3% 16S rRNA gene sequence similarity), Rhodopila globiformis (92.2-93.3%), and members of the genera Acidiphilium (91.3-93%) and Acidocella (91.8-92.4%). The DNA G+C contents of the novel strains were 60.5-61.9 mol%. The low levels of DNA-DNA relatedness (37%) and a number of phenotypic differences between the Siberian strains TPB606T and TPB621 and the tundra strain WM1T indicated that they represent two separate species. As the three isolates are clearly distinct from all recognized acidophilic members of the Alphaproteobacteria, they are considered to represent two novel species of a new genus, for which the names Acidisoma tundrae gen. nov., sp. nov. and Acidisoma sibiricum sp. nov. are proposed. The type strain of Acidisoma sibiricum is TPB606T (=DSM 21000T=VKM B-2487T) and the type strain of Acidisoma tundrae is WM1T (=DSM 19999T=VKM B-2488T).
从俄罗斯酸性泥炭藓为主的冻原和西伯利亚湿地中分离出三株专性需氧、异养细菌，分别命名为WM1T、TPB606T和TPB621菌株。这些分离菌株的细胞为革兰氏阴性、无运动性的球杆菌，单个、成对或成链状存在，且被大的荚膜覆盖。这些新菌株是中度嗜酸和耐冷的生物，能够在pH 3.0 - 7.6和2 - 30℃下生长。细胞内含有大量聚-β-羟基丁酸颗粒（每个细胞3 - 4个）。主要细胞脂肪酸为环C19:0ω8c，主要醌为Q - 10。从西伯利亚湿地分离出的TPB606T和TPB621菌株具有几乎相同的16S rRNA基因序列，与冻原菌株WM1T的序列相似性为97.2%。这三株菌株被证明属于α-变形菌纲，但与嗜酸细菌红酸球囊菌（Acidisphaera rubrifaciens，16S rRNA基因序列相似性为93.4 - 94.3%）、球形红假单胞菌（Rhodopila globiformis，92.2 - 93.3%）以及嗜酸菌属（Acidiphilium，91.3 - 93%）和酸单胞菌属（Acidocella，91.8 - 92.4%）的模式菌株仅存在较远的亲缘关系。新菌株的DNA G + C含量为60.5 - 61.9 mol%。西伯利亚菌株TPB606T和TPB621与冻原菌株WM1T之间的DNA - DNA相关性较低（37%）以及一些表型差异表明它们代表两个不同的物种。由于这三株分离菌株明显不同于α-变形菌纲中所有已被认可的嗜酸成员，它们被认为代表一个新属的两个新物种，为此提出了冻土酸菌属（Acidisoma）新属名，新种名为冻土酸菌（Acidisoma tundrae）和西伯利亚酸菌（Acidisoma sibiricum）。西伯利亚酸菌的模式菌株是TPB606T（=DSM 21000T =VKM B - 2487T），冻土酸菌的模式菌株是WM1T（=DSM 19999T =VKM B - 2488T）。
