局部应用δ-氨基乙酰丙酸后，上皮性皮肤肿瘤和银屑病皮损中卟啉的最佳蓄积情况。
Optimum porphyrin accumulation in epithelial skin tumours and psoriatic lesions after topical application of delta-aminolaevulinic acid.
作者信息
Fritsch C, Lehmann P, Stahl W, Schulte K W, Blohm E, Lang K, Sies H, Ruzicka T
机构信息
Institute of Physiological Chemistry I, and Department of Dermatology, Heinrich-Heine-University Düsseldorf, Germany.
出版信息
Br J Cancer. 1999 Mar;79(9-10):1603-8. doi: 10.1038/sj.bjc.6690255.
Photodynamic therapy with topically applied delta-aminolaevulinic acid is used to treat skin tumours by employing endogenously formed porphyrins as photosensitizers. This study examines the time course of porphyrin metabolite formation after topical application of delta-aminolaevulinic acid. Porphyrin biosynthesis in human skin tumours (basal cell carcinoma, squamous cell carcinoma), in psoriatic lesions, and in normal skin was investigated. Skin areas were treated with delta-aminolaevulinic acid, and levels of total porphyrins, porphyrin metabolites and proteins were measured in samples excised after 1, 2, 4, 6, 9, 12 and 24 h. There was an increase in porphyrin biosynthesis in all tissues with maximum porphyrin levels in tumours between 2 and 6 h and in psoriatic lesions 6 h after treatment. The pattern of porphyrins showed no significant difference between normal and neoplastic skin, protoporphyrin being the predominant metabolite. The results suggest that optimum irradiation time for superficial epithelial skin tumours may be as soon as 2 h after application of delta-aminolaevulinic acid, whereas for treatment of psoriatic lesions an application time of 6 h is more suitable.
外用δ-氨基乙酰丙酸的光动力疗法通过利用内源性形成的卟啉作为光敏剂来治疗皮肤肿瘤。本研究检测了外用δ-氨基乙酰丙酸后卟啉代谢产物的形成时间过程。对人类皮肤肿瘤（基底细胞癌、鳞状细胞癌）、银屑病皮损及正常皮肤中的卟啉生物合成进行了研究。皮肤区域用δ-氨基乙酰丙酸处理，在处理后1、2、4、6、9、12和24小时切除的样本中测量总卟啉、卟啉代谢产物和蛋白质的水平。所有组织中的卟啉生物合成均增加，肿瘤中卟啉水平在处理后2至6小时达到最高，银屑病皮损在处理后6小时达到最高。正常皮肤和肿瘤性皮肤之间的卟啉模式无显著差异，原卟啉是主要代谢产物。结果表明，浅表上皮性皮肤肿瘤的最佳照射时间可能在应用δ-氨基乙酰丙酸后2小时，而对于银屑病皮损的治疗，6小时的应用时间更合适。
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