Peng Q, Warloe T, Moan J, Heyerdahl H, Steen H B, Nesland J M, Giercksky K E
Department of Pathology, Norwegian Radium Hospital, Montebello, Oslo, Norway.
Photochem Photobiol. 1995 Nov;62(5):906-13. doi: 10.1111/j.1751-1097.1995.tb09154.x.
Microscopic fluorescence photometry incorporating a light-sensitive thermo-electrically cooled charge-coupled device (CCD) camera was employed to investigate the fluorescence distribution of 5-aminolevulinic acid (ALA)-induced porphyrins in 22 patients with a total number of 52 noduloulcerative basal cell carcinomas (BCC) after topical ALA application with or without dimethylsulfoxide (DMSO)/ethylenediaminetetraacetic acid (EDTA) or after intravenous administration of ALA. Both localization patterns and amounts of ALA-induced porphyrins in the BCC were studied. The ALA-induced porphyrins were localized only in the superficial layers of the noduloulcerative BCC lesions after topical application of 20% ALA alone for 3 h. However, both the penetration of ALA into deep lesions and the production of the ALA-induced porphyrin fluorescence were increased after topical administration of 20% ALA and 20% DMSO/4% EDTA for 3 h. Prior treatment with 99% DMSO for 15 min further enhanced the ALA penetration into the BCC lesions after topical application of the ALA/DMSO/EDTA mixture and produced more ALA-induced porphyrins by a factor of about three compared with those treated with ALA alone. The penetration of ALA into the deep BCC lesions could also be increased by prolonging the time of topical application of 20% ALA/4% EDTA to 29-48 h (without DMSO). Intravenous injection of ALA led to a more homogeneous distribution of the ALA-derived porphyrins in the whole noduloulcerative BCC lesions.
采用配备了光敏感热电冷却电荷耦合器件（CCD）相机的显微荧光光度法，研究了22例患者共52个结节溃疡性基底细胞癌（BCC）在局部应用5-氨基乙酰丙酸（ALA）（有或无二甲基亚砜（DMSO）/乙二胺四乙酸（EDTA））或静脉注射ALA后，ALA诱导的卟啉的荧光分布。对BCC中ALA诱导的卟啉的定位模式和含量均进行了研究。单独局部应用20%ALA 3小时后，ALA诱导的卟啉仅定位在结节溃疡性BCC病变的表层。然而，局部应用20%ALA和20%DMSO/4%EDTA 3小时后，ALA向深部病变的渗透以及ALA诱导的卟啉荧光的产生均增加。在局部应用ALA/DMSO/EDTA混合物之前，先用99%DMSO处理15分钟，可进一步增强ALA向BCC病变的渗透，与单独用ALA处理的相比，产生的ALA诱导的卟啉量增加约三倍。将20%ALA/4%EDTA的局部应用时间延长至29 - 48小时（无DMSO），也可增加ALA向深部BCC病变的渗透。静脉注射ALA导致ALA衍生的卟啉在整个结节溃疡性BCC病变中分布更均匀。
