Barshack I, Kopolovic J, Malik Z, Rothmann C
Pathology Department, Sheba Medical Center, Tel-Hashomer, Israel.
Br J Cancer. 1999 Mar;79(9-10):1613-9. doi: 10.1038/sj.bjc.6690257.
The spectral morphometric characteristics of standard haematoxylin and eosin breast carcinoma specimens were evaluated by light microscopy combined with a spectral imaging system. Light intensity at each wavelength in the range of 450-800 nm was recorded for 10(4) pixels from each field and represented as transmitted light spectra. A library of six characteristic spectra served to scan the cells and reconstruct new images depicting the nuclear area occupied by each spectrum. Fifteen cases of infiltrating ductal carcinoma and six cases of lobular carcinoma were examined; nine of the infiltrating ductal carcinoma and three of the lobular carcinoma showed an in situ component. The spectral morphometric analysis revealed a correlation between specific patterns of spectra and different groups of breast carcinoma cells. The most consistent result was that lobular carcinoma cells of in situ and infiltrating components from all patients showed a similar spectral pattern, whereas ductal carcinoma cells displayed spectral variety. Comparison of the in situ and the infiltrating ductal solid, cribriform and comedo carcinoma cells from the same patient revealed a strong similarity of the spectral elements and their relative distribution in the nucleus. The spectrum designated as number 5 in the library incorporated more than 40% of the nuclear area in 74.08% of the infiltrating lobular cells and in 13.64% of the infiltrating ductal carcinoma cells (P < 0.001). Spectrum number 2 appeared in all infiltrating ductal cells examined and in none of the lobular cells. These results indicate that spectrum number 5 is related to infiltrating lobular carcinoma, whereas spectrum number 2 is characteristic for infiltrating ductal carcinoma cells. Spectral similarity mapping of central necrotic regions of comedo type in situ carcinoma revealed nuclear fragmentation into defined segments composed of highly condensed chromatin. We conclude that the spectral morphometric features found for lobular and ductal cell populations may serve future automated histological diagnostics.
采用光学显微镜结合光谱成像系统，对标准苏木精和伊红染色的乳腺癌标本的光谱形态特征进行了评估。在450 - 800nm范围内，从每个视野的10⁴个像素记录每个波长的光强度，并表示为透射光谱。一个包含六种特征光谱的库用于扫描细胞并重建描绘每个光谱所占据核区域的新图像。检查了15例浸润性导管癌和6例小叶癌；其中9例浸润性导管癌和3例小叶癌显示有原位成分。光谱形态分析揭示了特定光谱模式与不同组乳腺癌细胞之间的相关性。最一致的结果是，所有患者原位和浸润成分的小叶癌细胞显示出相似的光谱模式，而导管癌细胞则表现出光谱多样性。对同一患者的原位和浸润性导管实性、筛状和粉刺样癌细胞进行比较，发现光谱元素及其在细胞核中的相对分布具有很强的相似性。在库中被指定为5号的光谱在74.08%的浸润性小叶细胞和13.64%的浸润性导管癌细胞中占据了超过40%的核面积（P < 0.001）。2号光谱出现在所有检查的浸润性导管细胞中，而在小叶细胞中均未出现。这些结果表明，5号光谱与浸润性小叶癌相关，而2号光谱是浸润性导管癌细胞的特征。粉刺样原位癌中央坏死区域的光谱相似性映射显示细胞核碎裂成由高度浓缩染色质组成的明确片段。我们得出结论，小叶和导管细胞群体的光谱形态特征可能有助于未来的自动化组织学诊断。
