Park Meeja, Baek Taehwa, Baek Jongho, Son Hyunjin, Kang Dongwook, Kim Jooheon, Lee Hyekyung
Department of Pathology, Eulji University Hospital, Eulji University School of Medicine, Daejeon, Korea.
Korean J Pathol. 2012 Feb;46(1):38-41. doi: 10.4132/KoreanJPathol.2012.46.1.38. Epub 2012 Feb 23.
There is a subjective disagreement about nuclear chromatin in the field of pathology. Objective values of red, green, and blue (RGB) light intensities for nuclear chromatin can be obtained through a quantitative analysis using digital images.
We examined 10 cases of well differentiated neuroendocrine tumors of the rectum, small cell lung carcinomas, and moderately differentiated squamous cell lung carcinomas respectively. For each case, we selected 30 representative cells and captured typical microscopic findings. Using an image analyzer, we determined the longest nuclear line profiles and obtained graph files and Excel data on RGB light intensities. We assessed the meaningful differences in graph files and Excel data among the three different tumors.
The nucleus of hematoxylin and eosin-stained tumor cells was expressed as a combination of RGB light sources. The highest intensity was from blue, whereas the lowest intensity was from green. According to the graph files, green showed the most noticeable change in the light intensity, which is consistent with the difference in standard deviations.
The change in the light intensity for green has an important implication for differentiating between tumors. Specific features of the nucleus can be expressed in specific values of RGB light intensities.
病理学领域对于核染色质存在主观分歧。通过使用数字图像进行定量分析，可以获得核染色质红色、绿色和蓝色（RGB）光强度的客观值。
我们分别检查了10例直肠高分化神经内分泌肿瘤、小细胞肺癌和中分化肺鳞状细胞癌。对于每例病例，我们选择30个代表性细胞并获取典型的显微镜检查结果。使用图像分析仪，我们确定了最长的核线轮廓，并获得了关于RGB光强度的图形文件和Excel数据。我们评估了三种不同肿瘤在图形文件和Excel数据方面的有意义差异。
苏木精和伊红染色的肿瘤细胞核表现为RGB光源的组合。最高强度来自蓝色，而最低强度来自绿色。根据图形文件，绿色在光强度方面显示出最明显的变化，这与标准差的差异一致。
绿色光强度的变化对于区分肿瘤具有重要意义。细胞核的特定特征可以通过RGB光强度的特定值来表达。
