Schröck E, du Manoir S, Veldman T, Schoell B, Wienberg J, Ferguson-Smith M A, Ning Y, Ledbetter D H, Bar-Am I, Soenksen D, Garini Y, Ried T
Diagnostic Development Branch, National Center for Human Genome Research, National Institutes of Health, Bethesda, MD 20892-4470, USA.
Science. 1996 Jul 26;273(5274):494-7. doi: 10.1126/science.273.5274.494.
The simultaneous and unequivocal discernment of all human chromosomes in different colors would be of significant clinical and biologic importance. Whole-genome scanning by spectral karyotyping allowed instantaneous visualization of defined emission spectra for each human chromosome after fluorescence in situ hybridization. By means of computer separation (classification) of spectra, spectrally overlapping chromosome-specific DNA probes could be resolved, and all human chromosomes were simultaneously identified.
同时且明确无误地以不同颜色分辨所有人的染色体具有重大的临床和生物学意义。通过光谱核型分析进行全基因组扫描，在荧光原位杂交后能够即时观察到每个人类染色体特定的发射光谱。借助光谱的计算机分离（分类），可以分辨光谱重叠的染色体特异性DNA探针，从而同时识别所有人类染色体。
