Niemeyer G, Kueng N
Department of Ophthalmology, University Hospital Zurich, Switzerland.
Doc Ophthalmol. 1998;95(1):55-61. doi: 10.1023/a:1001776428445.
We describe the fabrication of a simple silver-silk electrode which permits remarkably stable d.c.recording of the electroretinogram (ERG) and the optic nerve response (ONR). A saline soaked wick of surgical silk, guided into a polyethylene tube connects the tissue to a coil of Ag/AgCl wire placed in a small glass vial, which is filled with 0.9% NaCl. The vial that holds the tube and the wire is closed with a rubber cap allowing easy refilling with NaCl. Examples of the usefulness of the new silver-silk electrode are shown. We applied it in experimental work in the isolated arterially perfused cat eye for d.c.recordings of the ERG and the optic nerve response (ONR), and also in vivo, in anesthetized mice to record c-waves.
我们描述了一种简单的银丝电极的制作方法，该电极能够非常稳定地直流记录视网膜电图（ERG）和视神经反应（ONR）。将一根浸有生理盐水的手术丝线芯引入聚乙烯管中，连接组织与置于小玻璃瓶中的Ag/AgCl丝线圈，小玻璃瓶中装有0.9%的NaCl。装有管子和金属丝的小瓶用橡胶帽封闭，便于重新填充NaCl。展示了新型银丝电极的实用性示例。我们将其应用于离体动脉灌注猫眼的实验工作中，用于直流记录ERG和视神经反应（ONR），还在活体麻醉小鼠中记录c波。
