Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 108, PO Box 80154, 3508 TD Utrecht, The Netherlands.
Vet Microbiol. 2010 Jul 29;144(1-2):147-52. doi: 10.1016/j.vetmic.2009.12.046. Epub 2010 Feb 1.
The periodontal pathogens Porphyromonas gingivalis and Tannerella forsythia are strongly associated with periodontal disease and are highly prevalent in humans with periodontitis. Porphyromonas and Tannerella spp. have also been isolated from the oral cavity of cats. The oral microflora in animals was compared with those in humans in earlier studies, but no studies are available on the comparison of the oral microflora from pets and their respective owners. The aim of this study was to determine the presence of these bacteria in the oral microflora of cats and their owners, since animal to human transmission, or vice versa, of oral pathogens could have public health implications. This study investigated the prevalence of Porphyromonas gulae, P. gingivalis, and T. forsythia in the oral microflora of cats and their owners, using culture and polymerase chain reaction (PCR). All Porphyromonas isolates from cats (n=64) were catalase positive, whereas the Porphyromonas isolates from owners (n=7) were catalase negative, suggesting that the isolates from cats were P. gulae whereas those from the owners were P. gingivalis. T. forsythia was recovered from both cats (n=63) and owners (n=31); the proportion of T. forsythia relative to the total CFU was higher in cats with periodontitis than in cats without periodontal disease. Genotyping of T. forsythia isolates (n=54) in six cat/owner couples showed that in one cat/owner couple the T. forsythia isolates (n=6) were identical. These T. forsythia isolates were all catalase positive, which led us to hypothesize that transmission from cats to owners had occurred and that cats may be a reservoir of T. forsythia.
牙龈卟啉单胞菌和福赛斯坦纳菌是牙周病的主要病原体，在患有牙周炎的人群中高度流行。牙龈卟啉单胞菌和福赛斯坦纳菌也已从猫的口腔中分离出来。在早期的研究中，曾比较过动物的口腔微生物群与人类的口腔微生物群，但目前尚无关于宠物及其各自主人的口腔微生物群比较的研究。本研究旨在确定这些细菌是否存在于猫和其主人的口腔微生物群中，因为口腔病原体从动物传播到人，或者反之，可能会对公共卫生产生影响。本研究通过培养和聚合酶链反应（PCR）调查了猫和其主人的口腔微生物群中牙龈卟啉单胞菌、牙龈卟啉单胞菌和福赛斯坦纳菌的流行情况。从猫（n=64）中分离的所有牙龈卟啉单胞菌均为过氧化氢酶阳性，而从主人（n=7）中分离的牙龈卟啉单胞菌为过氧化氢酶阴性，表明从猫中分离的细菌为牙龈卟啉单胞菌，而从主人中分离的细菌为牙龈卟啉单胞菌。福赛斯坦纳菌从猫（n=63）和主人（n=31）中均有回收；患有牙周炎的猫口腔中的福赛斯坦纳菌相对总 CFU 的比例高于没有牙周病的猫。在六对猫/主人的福赛斯坦纳菌分离株（n=54）中进行基因分型显示，在一对猫/主人中，福赛斯坦纳菌分离株（n=6）相同。这些福赛斯坦纳菌分离株均为过氧化氢酶阳性，这使我们假设从猫到主人的传播已经发生，并且猫可能是福赛斯坦纳菌的储主。
