Waechter A I, Cavé A, Hocquemiller R, Bories C, Muñoz V, Fournet A
Laboratoire de Pharmacognosie, BIOCIS URA 1843 CNRS, Faculté de Pharmacie, Châtenay-Malabry, France.
Phytother Res. 1999 Mar;13(2):175-7. doi: 10.1002/(SICI)1099-1573(199903)13:2<175::AID-PTR395>3.0.CO;2-N.
On a preliminary screening, substantial leishmanicidal activity was observed for the petroleum ether and alkaloidal extracts of the stem bark of Unonopsis buchtienii, the alkaloids and sterols isolated from these were studied. Of the alkaloids, liriodenine exhibited the highest activity against Leishmania major and L donovani (IC100 = 3.12 micrograms/mL). On the other hand, O-methylmoschatoline and the petroleum ether extract without alkaloids showed an interesting in vitro activity against Trypanosoma brucei with an IC100 of 6.25 micrograms/mL. The highest cytotoxic activities were found with the petroleum ether extracts without alkaloids and with all alkaloids isolated (IC50 < 9 micrograms/mL for Vero cell line).
在初步筛选中，观察到布氏紫玉盘茎皮的石油醚提取物和生物碱提取物具有显著的杀利什曼原虫活性，并对从中分离出的生物碱和甾醇进行了研究。在生物碱中，鹅掌楸碱对硕大利什曼原虫和杜氏利什曼原虫表现出最高活性（IC100 = 3.12微克/毫升）。另一方面，O-甲基番荔枝碱和不含生物碱的石油醚提取物对布氏锥虫显示出有趣的体外活性，IC100为6.25微克/毫升。不含生物碱的石油醚提取物和所有分离出的生物碱具有最高的细胞毒性活性（Vero细胞系的IC50 < 9微克/毫升）。
