Pham L, Dahiya R, Rubinsky B
Bioengineering Laboratory, Department of Mechanical Engineering, University of California, Berkeley, California 94720, USA.
Cryobiology. 1999 Mar;38(2):169-75. doi: 10.1006/cryo.1999.2158.
Cryosurgery employs freezing to destroy undesirable tissue. However, under certain thermal conditions, frozen tissues survive. The survival of frozen undesirable tissue may lead to complications, such as recurrence of cancer. In a study of nude mice with subcutaneous metastatic prostate tumors, we showed that the preoperative injection of a phosphate-buffered saline solution with 10 mg/ml antifreeze protein of type I into the tumor prior to freezing enhances destruction under thermal conditions which normally yield cell survival. This suggests that the adjunctive use of antifreeze proteins in cryosurgery may reduce the complications from undesirable tissues that survive freezing.
冷冻手术利用冷冻来破坏不需要的组织。然而，在某些热条件下，冷冻的组织会存活下来。冷冻的不需要的组织存活可能会导致并发症，比如癌症复发。在一项对患有皮下转移性前列腺肿瘤的裸鼠的研究中，我们发现，在冷冻前向肿瘤中预先注射含10毫克/毫升I型抗冻蛋白的磷酸盐缓冲盐溶液，可在通常会使细胞存活的热条件下增强破坏效果。这表明在冷冻手术中辅助使用抗冻蛋白可能会减少因冷冻后存活的不需要的组织而引发的并发症。
