Uylings H B, Zilles K, Rajkowska G
Department of Anatomy, Vrije Universiteit, Meibergdreef 33, Amsterdam, 1105 AZ, The Netherlands.
Neuroimage. 1999 Apr;9(4):439-45. doi: 10.1006/nimg.1999.0417.
For cytoarchitectonic delineation of cortical areas in human brain, the Gallyas staining for somata with its sharp contrast between cell bodies and neuropil is preferable to the classical Nissl staining, the more so when an image analysis system is used. This Gallyas staining, however, does not appear to be appropriate for counting neuron numbers in pertinent brain areas, due to the lack of distinct cytological features between small neurons and glial cells. For cell counting Nissl is preferable. In an optimal design for cell counting at least both the Gallyas and the Nissl staining must be applied, the former staining for cytoarchitectural delineaton of cortical areas and the latter for counting the number of neurons in the pertinent cortical areas.
对于人类大脑皮质区域的细胞构筑学描绘，用于胞体的Gallyas染色在细胞体与神经毡之间具有鲜明对比，比经典的尼氏染色更可取，当使用图像分析系统时更是如此。然而，由于小神经元和神经胶质细胞之间缺乏明显的细胞学特征，这种Gallyas染色似乎不适用于对相关脑区的神经元进行计数。对于细胞计数，尼氏染色更可取。在细胞计数的最佳设计中，至少必须同时应用Gallyas染色和尼氏染色，前者用于皮质区域的细胞构筑学描绘，后者用于计算相关皮质区域的神经元数量。
