Pereira B, Bechara E J, Mendonça J R, Curi R
Department of Biodynamic, Escola de Educação Fisica e Esportes, São Paulo, Brazil.
Cell Biochem Funct. 1999 Mar;17(1):15-9. doi: 10.1002/(SICI)1099-0844(199903)17:1<15::AID-CBF804>3.0.CO;2-T.
The effect of dexamethasone (a synthetic glucocorticoid) on the activity of antioxidant enzymes (superoxide dismutase (SOD), catalase and glutathione peroxidase) of the lymphoid organs (mesenteric lymph nodes (MLN), spleen and thymus) was investigated. For comparison with non-immune tissues, skeletal muscles (soleus and gastrocnemius (GC) were also studied. As an indication of the occurrence of lipid peroxidation, the content of thiobarbituric acid reactant substances (TBARs) was also determined. Dexamethasone treatment decreased the TBARs content of the lymphoid organs and raised it in the GC and soleus muscles. The activity of Cu/Zn-SOD was reduced in all tissues. However, the activity of Mn-SOD was decreased in the MLN and soleus muscle only. The activity of catalase was reduced in the MLN and thymus and raised in the spleen and GC and soleus muscles. The imposed treatment raised the activity of GPX in the MLN, thymus and spleen and reduced it in GC and soleus muscles. These data led us to postulate that the mechanism for the therapeutic effect of glucocorticoids as antiinflammatory and immunosuppressive agents might include modification of antioxidant enzyme activities.
研究了地塞米松（一种合成糖皮质激素）对淋巴器官（肠系膜淋巴结（MLN）、脾脏和胸腺）中抗氧化酶（超氧化物歧化酶（SOD）、过氧化氢酶和谷胱甘肽过氧化物酶）活性的影响。为了与非免疫组织进行比较，还研究了骨骼肌（比目鱼肌和腓肠肌（GC））。作为脂质过氧化发生的指标，还测定了硫代巴比妥酸反应物（TBARs）的含量。地塞米松处理降低了淋巴器官中TBARs的含量，而提高了GC和比目鱼肌中TBARs的含量。所有组织中铜/锌超氧化物歧化酶（Cu/Zn-SOD）的活性均降低。然而，仅在MLN和比目鱼肌中锰超氧化物歧化酶（Mn-SOD）的活性降低。过氧化氢酶的活性在MLN和胸腺中降低，而在脾脏、GC和比目鱼肌中升高。所施加的处理提高了MLN、胸腺和脾脏中谷胱甘肽过氧化物酶（GPX）的活性，而降低了GC和比目鱼肌中GPX的活性。这些数据使我们推测，糖皮质激素作为抗炎和免疫抑制剂的治疗作用机制可能包括抗氧化酶活性的改变。
