经口灌喂鱼油对21日龄孕鼠淋巴器官抗氧化酶活性的影响。

Changes in the activities of antioxidant enzymes of the lymphoid organs of 21-day pregnant rats due to administration of fish oil by gavage.

De Souza J A, De Oliveria H R, Miyasaka C K, Gacek F, Torres R P, Mancini Filho J, Curi R

Department of Physiology and Biophysics, University of São Paulo, Brazil.

Gen Pharmacol. 1997 Oct;29(4):551-5. doi: 10.1016/s0306-3623(96)00576-9.

Abstract

The effect of fish oil administration by gavage (0.4% body weight) on activities of superoxide dismutase (SOD), catalase and glutathione peroxidase (GSH-Px) and on content of thiobarbituric acid reactive substances (TBARs) of the lymphoid organs [thymus, spleen and mesenteric lymph nodes (MLN)] and liver was investigated in 21-day pregnant rats. The results were compared with those obtained by administration of soybean oil, cocoa butter and coconut oil. 2. Oil administration did not have any significant effect on antioxidant enzyme activities of the liver, whereas marked changes were found in the lymphoid organs. The MLN presented the most pronounced changes: SOD and catalase activities were increased by the four oils; GSH-Px activity was raised by soybean and fish oils; coconut oil reduced the activity of the three antioxidant enzymes in this organ. 3. Fish oil given by gavage does affect the antioxidant capacity of the lymphoid organs; however, similar effect was also observed for cocoa butter and soybean oil. These changes in the antioxidant enzyme activities were able to prevent the lipid peroxidation process in the lymphoid organs.

摘要

对21天孕期的大鼠经口灌胃给予鱼油（0.4%体重），研究其对淋巴器官（胸腺、脾脏和肠系膜淋巴结）及肝脏中超氧化物歧化酶（SOD）、过氧化氢酶和谷胱甘肽过氧化物酶（GSH-Px）活性以及硫代巴比妥酸反应性物质（TBARs）含量的影响。将结果与给予大豆油、可可脂和椰子油所获得的结果进行比较。2. 给予油脂对肝脏的抗氧化酶活性没有任何显著影响，而在淋巴器官中发现了明显变化。肠系膜淋巴结呈现出最显著的变化：四种油脂均使SOD和过氧化氢酶活性增加；大豆油和鱼油使GSH-Px活性升高；椰子油降低了该器官中三种抗氧化酶的活性。3. 经口灌胃给予鱼油确实会影响淋巴器官的抗氧化能力；然而，可可脂和大豆油也观察到了类似的效果。抗氧化酶活性的这些变化能够防止淋巴器官中的脂质过氧化过程。