Savarino A, Calosso L, Piragino A, Martini C, Gennero L, Pescarmona G P, Pugliese A
Department of Medical and Surgical Sciences, University of Turin, Italy.
Cell Biochem Funct. 1999 Mar;17(1):47-55. doi: 10.1002/(SICI)1099-0844(199903)17:1<47::AID-CBF810>3.0.CO;2-V.
To investigate whether transferrin receptor (CD71) expression is affected by acute HIV-1 infection, three different lymphoid cell lines (MT-4, SUPT-1, H9) were infected with HIV-1 and tested for surface CD71 expression after different incubation periods depending on cell survival after infection. We found that expression of surface CD71 was lower in cells infected with HIV-1 than in uninfected controls: the timing and extent of this down-modulation depended apparently on the different susceptibility of the cell lines to HIV-1 infection and cytopathogenicity. Citrate, a molecule capable of chelating iron, dose-dependently prevented down-modulation of surface CD71 in HIV-1 infected cells as well as viral cytopathic effects. We conclude that (i) expression of surface transferrin receptors is down-modulated by acute HIV-1 infection in T lymphoid cells, that (ii) this cell phenotypic modulation is associated with the cytopathic effects of the virus, and that (iii) these phenomena are modulated by iron chelation. These results support the view that iron metabolism may be an important area for interaction between HIV-1 and human cells.
为研究转铁蛋白受体（CD71）表达是否受急性HIV-1感染的影响，用HIV-1感染三种不同的淋巴细胞系（MT-4、SUPT-1、H9），并根据感染后细胞存活情况在不同孵育期检测表面CD71表达。我们发现，感染HIV-1的细胞中表面CD71的表达低于未感染的对照细胞：这种下调的时间和程度显然取决于细胞系对HIV-1感染和细胞致病性的不同易感性。柠檬酸盐是一种能够螯合铁的分子，它能剂量依赖性地阻止HIV-1感染细胞中表面CD71的下调以及病毒的细胞病变效应。我们得出结论：（i）在T淋巴细胞中，急性HIV-1感染可下调表面转铁蛋白受体的表达；（ii）这种细胞表型调节与病毒的细胞病变效应相关；（iii）这些现象可通过铁螯合进行调节。这些结果支持了铁代谢可能是HIV-1与人类细胞相互作用的一个重要领域的观点。
