Tilt N, Hamilton M A
Montana State University, Bozeman 59717-3980, USA.
J AOAC Int. 1999 Mar-Apr;82(2):384-9.
The results of a quantitative antimicrobial assay can be summarized by the log reduction value. For an assay to be proposed as a standard method, it is usually necessary to conduct a collaborative study to demonstrate that the repeatability and reproducibility standard deviations (SDs) of the log reduction values are sufficiently small. It is not clear, however, precisely how small those SDs should be. This paper describes the results of a literature review conducted to determine the range of repeatability and reproducibility SDs for standard quantitative antimicrobial assays. The underlying premise is that, for an assay to have been accepted as a standard method, its repeatability and reproducibility SDs must have been sufficiently small. This premise implies that the repeatability and reproducibility SDs of standard assays establish de facto guidelines for acceptability. The survey comprised papers where the SDs could be extracted directly or where they could be calculated from accessible data. Papers describing suspension tests as well as hard surface tests were included. For the standard antimicrobial assays reviewed, repeatability SDs ranged from 0.25 to 1.21 and the reproducibility SDs ranged from 0.31 to 1.54.
定量抗菌检测的结果可用对数减少值来概括。对于一项拟作为标准方法的检测，通常有必要开展协作研究，以证明对数减少值的重复性和再现性标准偏差（SDs）足够小。然而，这些标准偏差究竟应该多小尚不清楚。本文描述了为确定标准定量抗菌检测的重复性和再现性标准偏差范围而进行的文献综述结果。其基本前提是，对于一项已被接受为标准方法的检测，其重复性和再现性标准偏差必定足够小。这一前提意味着标准检测的重复性和再现性标准偏差实际上确立了可接受性的指导原则。该调查涵盖了能够直接提取标准偏差或可根据可获取数据计算标准偏差的论文。其中包括描述悬液试验以及硬表面试验的论文。对于所综述的标准抗菌检测，重复性标准偏差范围为0.25至1.21，再现性标准偏差范围为0.31至1.54。
