Bellón J M, Contreras L A, Pascual G, Bujan J
Department of Morphological Sciences and Surgery, Faculty of Medicine, University of Alcala de Henares, Madrid, Spain.
Eur J Surg. 1999 Feb;165(2):145-50. doi: 10.1080/110241599750007342.
To study the interfaces between the visceral peritoneum and some of the biomaterials used to repair defects in the abdominal wall.
Animal study.
School of medicine, Spain.
48 New Zealand white rabbits divided into 4 groups of 12 each.
Full thickness defects 50 x 70 mm were created in the abdominal wall and repaired with polytetrafluoroethylene (PTFE, Soft Tissue Patch), one of two polypropylene patches (Marlex and Prolene), or lyophylised dura mater (Lyo-Dura). 3 animals from each group were killed at 14, 30, 60 and 90 days and specimens examined by light microscopy, scanning electron microscopy, and immuno histochemistry by labelling of macrophages with RAM-11, a specific monoclonal antibody (MoAb).
Infection, healing, development of adhesions, and histological appearance of the interface.
Tissues responded similarly to materials of similar structures. Layered prostheses (PTFE and Lyo-Dura) caused formation of a well organised neoperitoneum with few adhesions to the abdominal viscera (loose adhesions in 2 animals in each group), whereas the mesh prostheses generated a disorganised neoperitoneum with many adhesions (Marlex loose adhesions 3, firm 8, and integrated 1; Prolene loose adhesions 2, firm 8, and integrated 2). Lyo-Dura was associated with the formation of areas of calcification. Labelling of macrophages with the MoAb showed that they were in direct contact with all materials studied.
Layered biomaterials with little or no porosity (PTFE and Lyo-Dura) are the most suitable of the four for implantation in sites where the prosthesis is in contact with the visceral peritoneum, because they induce minimal adhesions.
研究脏腹膜与一些用于修复腹壁缺损的生物材料之间的界面。
动物研究。
西班牙医学院。
48只新西兰白兔，分为4组，每组12只。
在腹壁制造50×70mm的全层缺损，并用聚四氟乙烯（PTFE，软组织补片）、两种聚丙烯补片（Marlex和Prolene）之一或冻干硬脑膜（Lyo - Dura）进行修复。每组分别于14、30、60和90天处死3只动物，标本进行光镜、扫描电镜检查，并用特异性单克隆抗体（MoAb）RAM - 11标记巨噬细胞进行免疫组织化学检查。
感染、愈合、粘连形成及界面的组织学表现。
组织对结构相似的材料反应相似。分层假体（PTFE和Lyo - Dura）导致形成组织良好的新腹膜，与腹腔脏器粘连较少（每组2只动物有疏松粘连），而网状假体产生的新腹膜结构紊乱，粘连较多（Marlex：疏松粘连3只，紧密粘连8只，融合1只；Prolene：疏松粘连2只，紧密粘连8只，融合2只）。Lyo - Dura与钙化区域的形成有关。用MoAb标记巨噬细胞显示它们与所有研究材料直接接触。
孔隙率小或无孔隙的分层生物材料（PTFE和Lyo - Dura）是四种材料中最适合植入假体与脏腹膜接触部位的，因为它们引起的粘连最少。
