Lin Y M, Flavin M T, Schure R, Chen F C, Sidwell R, Barnard D L, Huffman J H, Kern E R
MediChem Research, Lemont, Illinois, USA.
Planta Med. 1999 Mar;65(2):120-5. doi: 10.1055/s-1999-13971.
Biflavonoids such as amentoflavone (1), agathisflavone (2), robustaflavone (3), hinokiflavone (4), volkensiflavone (5), rhusflavanone (7), succedaneflavanone (9), all isolated from Rhus succedanea and Garcinia multiflora, as well as their methyl ethers and acetates, volkensiflavone hexamethyl ether (6), rhusflavanone hexaacetate (8), and succedaneflavanone hexaacetate (10) were evaluated for their antiviral activities. The inhibitory activities against a number of viruses including respiratory viruses (influenza A, influenza B, respiratory syncytial, parainfluenza type 3, adenovirus type 5, and measles) and herpes viruses (HSV-1, HSV-2, HCMV, and VZV) were investigated. The results indicated that robustaflavone exhibited strong inhibitory effects against influenza A and influenza B viruses with EC50 values of 2.0 micrograms/ml and 0.2 microgram/ml, respectively, and selectivity index values (SI) of 16 and 454, respectively. Amentoflavone and agathisflavone also demonstrated significant activity against influenza A and B viruses. Amentoflavone and robustaflavone exhibited moderate anti-HSV-1 anti-HSV-2 activities with EC50 values of 17.9 micrograms/ml (HSV-1) and 48.0 micrograms/ml (HSV-2) and SI values of > 5.6 (HSV-1) and > 2.1 (HSV-2) for amentoflavone; EC50 values of 8.6 micrograms/ml (HSV-1) and 8.5 micrograms/ml (HSV-2), and SI values of > 11.6 (HSV-1) and > 11.8 (HSV-2) for robustaflavone. Rhusflavanone demonstrated inhibitory activities against influenza B, measles, and HSV-2 viruses with SI values of 9.3, 8 and > 6.4, respectively. Succedaneaflavanone exhibited inhibitory activities against influenza B virus and VZV with SI values of 15 and < 3.0, respectively.
从盐肤木和多花山竹子中分离得到的双黄酮类化合物，如穗花杉双黄酮（1）、贝壳杉黄酮（2）、粗壮黄酮（3）、扁柏双黄酮（4）、伏康西黄酮（5）、盐肤木黄烷酮（7）、继盐肤木黄烷酮（9），以及它们的甲基醚和乙酸酯，伏康西黄酮六甲醚（6）、盐肤木黄烷酮六乙酸酯（8）和继盐肤木黄烷酮六乙酸酯（10），对它们的抗病毒活性进行了评估。研究了它们对多种病毒的抑制活性，包括呼吸道病毒（甲型流感病毒、乙型流感病毒、呼吸道合胞病毒、3型副流感病毒、5型腺病毒和麻疹病毒）和疱疹病毒（单纯疱疹病毒1型、单纯疱疹病毒2型、人巨细胞病毒和水痘带状疱疹病毒）。结果表明，粗壮黄酮对甲型流感病毒和乙型流感病毒表现出较强的抑制作用，其半数有效浓度（EC50）值分别为2.0微克/毫升和0.2微克/毫升，选择性指数（SI）值分别为16和454。穗花杉双黄酮和贝壳杉黄酮对甲型和乙型流感病毒也表现出显著活性。穗花杉双黄酮和粗壮黄酮对单纯疱疹病毒1型和单纯疱疹病毒2型表现出中等活性，穗花杉双黄酮的EC50值分别为17.9微克/毫升（单纯疱疹病毒1型）和48.0微克/毫升（单纯疱疹病毒2型），SI值分别>5.6（单纯疱疹病毒1型）和>2.1（单纯疱疹病毒2型）；粗壮黄酮的EC50值分别为8.6微克/毫升（单纯疱疹病毒1型）和8.5微克/毫升（单纯疱疹病毒2型），SI值分别>11.6（单纯疱疹病毒1型）和>11.8（单纯疱疹病毒2型）。盐肤木黄烷酮对乙型流感病毒、麻疹病毒和单纯疱疹病毒2型表现出抑制活性，SI值分别为9.3、8和>6.4。继盐肤木黄烷酮对乙型流感病毒和水痘带状疱疹病毒表现出抑制活性，SI值分别为15和<3.0。
