乙型肝炎表面抗原（HBsAg）慢性携带者血清中特异性IgA和IgM抗-HBc抗体的流行率与乙肝病毒DNA（HBV DNA）的比较
Prevalence of specific IgA and IgM anti-HBc antibodies compared with HBV DNA in the sera of HBsAg chronic carriers.
作者信息
Elsana S, Shemer-Avni Y, Sikuler E, Sarov B, Naggan L, Bar-Shany S, Gilon E, Yaari A, Orgel M, Frazer G M, Margalith M
机构信息
Department of Virology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
出版信息
J Hum Virol. 1997 Nov-Dec;1(1):52-7.
OBJECTIVE
We evaluated the significance of IgA antibodies directed against the hepatitis B virus core antigen (IgA anti-HBc) as a marker for viral replication.
STUDY DESIGN/METHODS: Serum samples of 143 hepatitis B surface antigen (HBsAg) carriers and 189 HBsAg-negative subjects were studied. Hepatitis B virus (HBV) DNA was detected by polymerase chain reaction. IgA anti-HBc was determined by a capture enzyme-linked immunosorbent assay developed in our laboratory. The results were compared with those for IgM anti-HBc, which were determined by a commercially available method.
RESULTS
IgA anti-HBc was detected in 57 (40%) and HBV DNA in 38 (27%) of the HBsAg carriers. Among the HBsAg-negative subjects, IgA anti-HBc and HBV DNA were detected simultaneously in four samples. All 42 HBV DNA-positive samples were IgA anti-HBc positive. IgM anti-HBc was detected in 27 (64%) of them.
CONCLUSIONS
IgA anti-HBc is a sensitive marker for HBV replication, and its absence may exclude HBV replication. The role of IgA anti-HBc in monitoring response to therapy and predicting clinical course is being evaluated.
目的
我们评估了针对乙肝病毒核心抗原的IgA抗体（IgA抗-HBc）作为病毒复制标志物的意义。
研究设计/方法：对143名乙肝表面抗原（HBsAg）携带者和189名HBsAg阴性受试者的血清样本进行了研究。采用聚合酶链反应检测乙肝病毒（HBV）DNA。IgA抗-HBc通过我们实验室开发的捕获酶联免疫吸附测定法进行测定。将结果与采用市售方法测定的IgM抗-HBc结果进行比较。
结果
在57名（40%）HBsAg携带者中检测到IgA抗-HBc，在38名（27%）中检测到HBV DNA。在HBsAg阴性受试者中，有4份样本同时检测到IgA抗-HBc和HBV DNA。所有42份HBV DNA阳性样本的IgA抗-HBc均为阳性。其中27份（64%）检测到IgM抗-HBc。
结论
IgA抗-HBc是HBV复制的敏感标志物，未检测到该抗体可排除HBV复制。目前正在评估IgA抗-HBc在监测治疗反应和预测临床病程中的作用。
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