Witt M, Kasper M
Department of Anatomy, Technical University Dresden, Germany.
Anat Embryol (Berl). 1999 Apr;199(4):291-9. doi: 10.1007/s004290050229.
Taste buds in humans originate from approximately the 8th postovulatory week under the influence of ingrowing nerve fibers. Since they develop from local epithelium, it is of interest whether or not prospective taste cells maintain or develop characteristics of epithelial cells that are different from those of the adjacent epithelium during differentiation. The aim of this study was to monitor changes of the distribution of the cytokeratin filaments (CKs) 8, 18, 19 and 20 ("gastrointestinal" type), CK 7 ("ductal" type), and CK 13 (maturation "mucosa type"), as well as vimentin in developing human taste buds and adjacent squamous epithelium. With the exception of CK13, which remains negative in taste bud anlagen and adult taste buds, all cytokeratins tested were present in taste cells. With the progress of development, the distribution of CKs becomes more and more restricted to taste cells and salivatory ducts as well as Ebner gland cells. Only CK20 is exclusively specific to taste bud anlagen and sometimes to individual bipolar cells occurring in early stages (week 8-9). Vimentin was located mainly in mesodermal derivatives but also in perigemmal epithelial cells during all stages of development. The occurrence of vimentin in "borderline" epithelia that interface with underlying connective tissue, i.e., in a region of discontinuity, may be associated with particular events in development, cell migration or even dedifferentiation.
人类味蕾在排卵后约第8周，在向内生长的神经纤维影响下起源。由于它们由局部上皮发育而来，因此在分化过程中，未来的味觉细胞是否保持或发展出与相邻上皮不同的上皮细胞特征，这一点很值得关注。本研究的目的是监测细胞角蛋白丝（CKs）8、18、19和20（“胃肠道”型）、CK 7（“导管”型）、CK 13（成熟“黏膜型”）以及波形蛋白在发育中的人类味蕾和相邻鳞状上皮中的分布变化。除了在味蕾原基和成年味蕾中保持阴性的CK13外，所有检测的细胞角蛋白都存在于味觉细胞中。随着发育的进行，CKs的分布越来越局限于味觉细胞、唾液腺导管以及埃布纳腺细胞。只有CK20仅对味蕾原基具有特异性，有时对早期（第8 - 9周）出现的单个双极细胞也具有特异性。波形蛋白主要位于中胚层衍生物中，但在发育的所有阶段也存在于味蕾周围的上皮细胞中。波形蛋白出现在与下方结缔组织交界的“边缘”上皮中，即在一个不连续区域，可能与发育、细胞迁移甚至去分化中的特定事件有关。
