Brand C U, Hunger R E, Yawalkar N, Gerber H A, Schaffner T, Braathen L R
Dermatological Clinic, University of Berne, Inselspital, Bern, Switzerland.
Arch Dermatol Res. 1999 Feb-Mar;291(2-3):65-72. doi: 10.1007/s004030050385.
The phenotype and function of CD1a+ lymph cells is of considerable interest. By means of microsurgical lymph cannulation human lymph derived from normal skin was sampled. Cells were isolated and processed for immunocytochemistry, electron microscopy, flow cytometry and functional assays. The majority of the cells, (62%), were T cells. The other cells comprised CD1a+ cells (7%), monocytes/macrophages (8%), and B cells (1%); the remainder were erythrocytes or uncharacterized cells. The CD1a+ cells reacted with antibodies against protein S-100, HLA-DR, the Lag antigen, CD4, CD11a, CD11b, CD18, CD25, CD40, CD54, CD80 and CD86. Interestingly, a small prolow portion the of CD1a+ cells (about 5%) reacted with an antibody to CD14. The CD1a+ cells did not react with an antibody against human follicular dendritic cells nor were they CD19-, CD23-, E-cadherin- or factor XIIIa-positive. Both allogenic and antigen-specific T cell proliferation stimulated by antigen-presenting lymph cells were strongly inhibited by adding anti-CD80 and anti-CD86 antibodies. By electron microscopy Birbeck granules were detected in only 22% of the CD1a+ lymph cells and these cells exhibited an extensive ruffling of the surface. These findings demonstrate that CD1a+ lymph cells, which do not express the dermal dendritic cell marker factor XIIIa, resemble dendritic cells formerly designated as 'veiled' as well as lymphoid dendritic cells, suggesting that after migration to the regional lymphoid organs, Langerhans cells form a more differentiated population of dendritic cells specialized in sensitizing T lymphocytes. Our results add further support to the view that resident Langerhans cells may be precursors of lymphoid dendritic cells acquiring the final phenotype in the microenvironment of the lymph node.
CD1a+淋巴细胞的表型和功能备受关注。通过显微外科淋巴管插管术采集了来自正常皮肤的人体淋巴液。分离细胞并进行免疫细胞化学、电子显微镜、流式细胞术和功能测定处理。大部分细胞（62%）为T细胞。其他细胞包括CD1a+细胞（7%）、单核细胞/巨噬细胞（8%）和B细胞（1%）；其余为红细胞或未鉴定细胞。CD1a+细胞与抗蛋白S-100、HLA-DR、Lag抗原、CD4、CD11a、CD11b、CD18、CD25、CD40、CD54、CD80和CD86的抗体发生反应。有趣的是，一小部分CD1a+细胞（约5%）与抗CD14抗体发生反应。CD1a+细胞不与抗人滤泡树突状细胞抗体发生反应，也不是CD19-、CD23-、E-钙黏蛋白-或因子XIIIa阳性。添加抗CD80和抗CD86抗体可强烈抑制抗原呈递淋巴细胞刺激的同种异体和抗原特异性T细胞增殖。通过电子显微镜检查，仅在22%的CD1a+淋巴细胞中检测到伯贝克颗粒，这些细胞表现出广泛的表面皱襞。这些发现表明，不表达真皮树突状细胞标志物因子XIIIa的CD1a+淋巴细胞类似于以前称为“面纱样”的树突状细胞以及淋巴样树突状细胞，这表明迁移到局部淋巴器官后，朗格汉斯细胞形成了一个更具分化性的树突状细胞群体，专门负责使T淋巴细胞致敏。我们的结果进一步支持了驻留朗格汉斯细胞可能是在淋巴结微环境中获得最终表型的淋巴样树突状细胞前体的观点。
