Stennicke H R, Salvesen G S
The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, California 92037, USA.
Methods. 1999 Apr;17(4):313-9. doi: 10.1006/meth.1999.0745.
Caspases and their involvement in programmed cell death have been an area of significant interest since their initial identification in 1992. To facilitate the search for new components involved in cell death, and to aid researchers in understanding the interactions between currently known cell death proteins, we describe a number of techniques commonly used in the preparation and characterization of caspases.
自1992年首次被鉴定以来，半胱天冬酶及其在程序性细胞死亡中的作用一直是一个备受关注的领域。为了便于寻找参与细胞死亡的新成分，并帮助研究人员理解目前已知的细胞死亡蛋白之间的相互作用，我们描述了一些常用于半胱天冬酶制备和特性鉴定的技术。
