Callahan L M, Vaules W A, Coleman P D
Department of Neurobiology & Anatomy, University of Rochester, New York 14642, USA.
J Neuropathol Exp Neurol. 1999 Mar;58(3):275-87. doi: 10.1097/00005072-199903000-00007.
Combining immunocytochemistry with in situ hybridization of Alzheimer disease (AD) hippocampus demonstrated a 50% reduction in grain density for synaptophysin message over CA1 pyramidal neurons containing neurofibrillary tangles (NFT) relative to near neighbor NFT-free neurons. This decrease was not global, but was selective since message grain density for the lysosomal protein, cathepsin D, increased 33% in these neurons (relative to NFT-free neurons). Poly A+ message grain density decreased by 25% in NFT neurons. Percent of the cell body containing NFT correlated -0.35 (p < 0.0001) with grain density for synaptophysin message. These data verify the concept of altered profiles of gene expression as a function of disease state within single cells and suggest that events associated with NFT formation may lead to altered expression of synaptic messages.
将免疫细胞化学与阿尔茨海默病（AD）海马体的原位杂交相结合，结果显示，与相邻无神经原纤维缠结（NFT）的神经元相比，含有神经原纤维缠结的CA1锥体神经元中突触素信息的颗粒密度降低了50%。这种减少并非全局性的，而是具有选择性，因为溶酶体蛋白组织蛋白酶D的信息颗粒密度在这些神经元中增加了33%（相对于无NFT的神经元）。NFT神经元中的多聚A+信息颗粒密度下降了25%。含有NFT的细胞体百分比与突触素信息的颗粒密度的相关性为-0.35（p < 0.0001）。这些数据证实了基因表达谱随单细胞疾病状态而改变的概念，并表明与NFT形成相关的事件可能导致突触信息表达的改变。
