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A region near the C-terminal end of Escherichia coli DNA helicase II is required for single-stranded DNA binding.大肠杆菌DNA解旋酶II的C末端附近的一个区域对于单链DNA结合是必需的。
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2
Site-directed mutations in motif VI of Escherichia coli DNA helicase II result in multiple biochemical defects: evidence for the involvement of motif VI in the coupling of ATPase and DNA binding activities via conformational changes.大肠杆菌DNA解旋酶II基序VI中的定点突变导致多种生化缺陷：基序VI通过构象变化参与ATP酶和DNA结合活性偶联的证据。
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3
Mutations in motif II of Escherichia coli DNA helicase II render the enzyme nonfunctional in both mismatch repair and excision repair with differential effects on the unwinding reaction.大肠杆菌DNA解旋酶II基序II中的突变使该酶在错配修复和切除修复中均失去功能，对解旋反应有不同影响。
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4
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本文引用的文献

1
The distribution of the numbers of mutants in bacterial populations.细菌群体中突变体数量的分布。
J Genet. 1949 Dec;49(3):264-85. doi: 10.1007/BF02986080.
2
Escherichia coli DNA helicase II is active as a monomer.大肠杆菌DNA解旋酶II以单体形式具有活性。
J Biol Chem. 1999 Apr 30;274(18):12488-98. doi: 10.1074/jbc.274.18.12488.
3
Site-directed mutations in motif VI of Escherichia coli DNA helicase II result in multiple biochemical defects: evidence for the involvement of motif VI in the coupling of ATPase and DNA binding activities via conformational changes.大肠杆菌DNA解旋酶II基序VI中的定点突变导致多种生化缺陷：基序VI通过构象变化参与ATP酶和DNA结合活性偶联的证据。
J Mol Biol. 1998 Mar 27;277(2):257-71. doi: 10.1006/jmbi.1997.1614.
4
Major domain swiveling revealed by the crystal structures of complexes of E. coli Rep helicase bound to single-stranded DNA and ADP.大肠杆菌Rep解旋酶与单链DNA及ADP复合物的晶体结构揭示的主要结构域旋转
Cell. 1997 Aug 22;90(4):635-47. doi: 10.1016/s0092-8674(00)80525-5.
5
Mutation of a highly conserved arginine in motif IV of Escherichia coli DNA helicase II results in an ATP-binding defect.大肠杆菌DNA解旋酶II基序IV中一个高度保守的精氨酸发生突变，导致ATP结合缺陷。
J Biol Chem. 1997 Jul 25;272(30):18614-20. doi: 10.1074/jbc.272.30.18614.
6
Biochemical analyses of mutations in the HSV-1 helicase-primase that alter ATP hydrolysis, DNA unwinding, and coupling between hydrolysis and unwinding.对单纯疱疹病毒1型解旋酶-引发酶中改变ATP水解、DNA解旋以及水解与解旋之间偶联的突变进行生化分析。
J Biol Chem. 1997 Feb 14;272(7):4623-30. doi: 10.1074/jbc.272.7.4623.
7
Human transcription-repair coupling factor CSB/ERCC6 is a DNA-stimulated ATPase but is not a helicase and does not disrupt the ternary transcription complex of stalled RNA polymerase II.人类转录修复偶联因子CSB/ERCC6是一种DNA刺激的ATP酶，但不是解旋酶，也不会破坏停滞的RNA聚合酶II的三元转录复合物。
J Biol Chem. 1997 Jan 17;272(3):1885-90. doi: 10.1074/jbc.272.3.1885.
8
A point mutation in Escherichia coli DNA helicase II renders the enzyme nonfunctional in two DNA repair pathways. Evidence for initiation of unwinding from a nick in vivo.大肠杆菌DNA解旋酶II中的一个点突变使该酶在两条DNA修复途径中失去功能。体内从切口开始解旋的证据。
J Biol Chem. 1997 Jan 3;272(1):572-9. doi: 10.1074/jbc.272.1.572.
9
Crystal structure of a DExx box DNA helicase.一种DExx盒DNA解旋酶的晶体结构。
Nature. 1996 Nov 28;384(6607):379-83. doi: 10.1038/384379a0.
10
Mechanisms of helicase-catalyzed DNA unwinding.解旋酶催化DNA解旋的机制。
Annu Rev Biochem. 1996;65:169-214. doi: 10.1146/annurev.bi.65.070196.001125.

大肠杆菌DNA解旋酶II的C末端附近的一个区域对于单链DNA结合是必需的。

A region near the C-terminal end of Escherichia coli DNA helicase II is required for single-stranded DNA binding.

作者信息

Mechanic L E, Latta M E, Matson S W

机构信息

Department of Biochemistry and Biophysics, Protein Engineering and Molecular Genetics Training Program, University of North Carolina, Chapel Hill, North Carolina 27599, USA.

出版信息

J Bacteriol. 1999 Apr;181(8):2519-26. doi: 10.1128/JB.181.8.2519-2526.1999.

DOI:10.1128/JB.181.8.2519-2526.1999
PMID:10198018
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC93680/
Abstract

The role of the C terminus of Escherichia coli DNA helicase II (UvrD), a region outside the conserved helicase motifs, was investigated by using three mutants: UvrDDelta107C (deletion of the last 107 C-terminal amino acids), UvrDDelta102C, and UvrDDelta40C. This region, which lacks sequence similarity with other helicases, may function to tailor UvrD for its specific in vivo roles. Genetic complementation assays demonstrated that mutant proteins UvrDDelta107C and UvrDDelta102C failed to substitute for the wild-type protein in methyl-directed mismatch repair and nucleotide excision repair. UvrDDelta40C protein fully complemented the loss of helicase II in both repair pathways. UvrDDelta102C and UvrDDelta40C were purified to apparent homogeneity and characterized biochemically. UvrDDelta102C was unable to bind single-stranded DNA and exhibited a greatly reduced single-stranded DNA-stimulated ATPase activity in comparison to the wild-type protein (kcat = 0.01% of the wild-type level). UvrDDelta40C was slightly defective for DNA binding and was essentially indistinguishable from wild-type UvrD when single-stranded DNA-stimulated ATP hydrolysis and helicase activities were measured. These results suggest a role for a region near the C terminus of helicase II in binding to single-stranded DNA.

摘要

利用三个突变体研究了大肠杆菌DNA解旋酶II（UvrD）的C末端（保守解旋酶基序之外的区域）的作用：UvrDDelta107C（删除最后107个C末端氨基酸）、UvrDDelta102C和UvrDDelta40C。该区域与其他解旋酶缺乏序列相似性，可能使UvrD适应其特定的体内作用。遗传互补分析表明，突变蛋白UvrDDelta107C和UvrDDelta102C在甲基化导向的错配修复和核苷酸切除修复中无法替代野生型蛋白。UvrDDelta40C蛋白在两种修复途径中完全弥补了解旋酶II的缺失。UvrDDelta102C和UvrDDelta40C被纯化至表观均一，并进行了生化特性分析。与野生型蛋白相比，UvrDDelta102C无法结合单链DNA，且单链DNA刺激的ATP酶活性大幅降低（kcat =野生型水平的0.01%）。当测量单链DNA刺激的ATP水解和解旋酶活性时，UvrDDelta40C在DNA结合方面略有缺陷，与野生型UvrD基本无差异。这些结果表明解旋酶II的C末端附近区域在结合单链DNA方面发挥作用。