Sakharova A V, Lozhnikova S M, Pirogov V N, Piradov M A
Research Institute of Neurology, Moscow.
Arkh Patol. 1999 Jan-Feb;61(1):39-46.
Distribution and intensity of NADPH-d reactivity, a marker for enzyme of the nitric oxide synthesis, in nervus suralis biopsies in severe DP were studied at light and electron microscopic levels. The study of control specimens has shown that NADPH-d reactivity was permanently present in Schwann cells (SC) and was distributed in all parts of their cytoplasm. Axon and myelin were devoid of NADPH-d reactivity. A decrease of enzyme reactivity in SC cytoplasm of the damaged nerve fibers and rising enzyme reactivity in the cytoplasm of activated SC were observed in DP. High reactivity in SC of small fibers was found at earlier stages and that of thick fibers at later stages. This distinction reflected, apparently, sequence of entering at first thin, then thick fibres in the reparative process. Under the electron microscope, the reaction product was deposited on membranes of endoplasmic reticulum, nuclear membrane and Golgi complex. The enzyme was also located in nucleus of activated SC. Ultrastructural location and the fact that the highest intensity of reaction is present in SC of nervous fibres with morphological signs of remyelination suggest link of this enzyme with the reparative process. This study provides the first evidence of NADPH-d reactivity in SC and shows that NADPH-d histochemistry is a useful tool for peripheral nerve biopsies study.
在光镜和电镜水平上，研究了严重糖尿病足（DP）腓肠神经活检中一氧化氮合成酶标志物NADPH-d反应性的分布和强度。对照标本研究表明，NADPH-d反应性始终存在于雪旺细胞（SC）中，并分布于其细胞质的所有部分。轴突和髓鞘无NADPH-d反应性。在DP中观察到受损神经纤维的SC细胞质中酶反应性降低，而活化SC细胞质中酶反应性升高。在早期发现小纤维的SC具有高反应性，后期发现粗纤维的SC具有高反应性。这种差异显然反映了修复过程中细纤维先进入、粗纤维后进入的顺序。在电子显微镜下，反应产物沉积在内质网、核膜和高尔基体复合体的膜上。该酶也位于活化SC的细胞核中。超微结构定位以及具有髓鞘再生形态学迹象的神经纤维的SC中存在最高反应强度这一事实表明该酶与修复过程有关。本研究首次提供了SC中NADPH-d反应性的证据，并表明NADPH-d组织化学是外周神经活检研究的有用工具。
