Moroz L L, Norekian T P, Pirtle T J, Robertson K J, Satterlie R A
The Whitney Laboratory, Department of Neuroscience, University of Florida, St. Augustine, Florida 32080-8623, USA.
J Comp Neurol. 2000 Nov 13;427(2):274-84.
The action of nitric oxide (NO) and the distribution of putative nitric oxide synthase-containing cells in the pelagic pteropod mollusc Clione limacina were studied using nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) histochemistry and conventional microelectrode techniques in the isolated central nervous system and in semi-intact preparations. The majority of NADPH-d-reactive neuronal somata were restricted to the cerebral ganglia. The labeled cells were small in diameter (20-30 microm) and were located in the medial areas of the ganglia. A pair of symmetrical neurons was found in the peripheral "olfactory organ." NADPH-d-reactive non-neuronal cells were detected in the periphery and were mainly associated with secretorylike cells and organs of the renopericardial system. The NO donor, diethylamine NO complex sodium salt (10-100 microM), activated neurons from both feeding and locomotory circuits. The cGMP analog, 8-Br-cGMP, mimicked the effects of NO on neurons. We suggest that NO is an endogenous neuromodulator involved in the control of some aspects of feeding and locomotor behavior of Clione.
利用烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）组织化学方法以及传统微电极技术，在分离的中枢神经系统和半完整标本中，研究了一氧化氮（NO）的作用以及远洋翼足类软体动物海若螺（Clione limacina）中假定含一氧化氮合酶细胞的分布。大多数NADPH-d反应性神经元胞体局限于脑神经节。标记的细胞直径较小（20 - 30微米），位于神经节的内侧区域。在外周“嗅觉器官 ”中发现了一对对称的神经元。在周围检测到NADPH-d反应性非神经元细胞，主要与肾心包系统的分泌样细胞和器官相关。NO供体二乙胺NO络合钠盐（10 - 100微摩尔）激活了摄食和运动回路中的神经元。环鸟苷酸类似物8 - 溴环鸟苷酸模仿了NO对神经元的作用。我们认为NO是一种内源性神经调节剂，参与控制海若螺摄食和运动行为的某些方面。
