Moroz Leonid L
The Whitney Laboratory for Marine Bioscience and Department of Neuroscience, University of Florida, St. Augustine, Florida 32080, USA.
J Comp Neurol. 2006 Mar 1;495(1):10-20. doi: 10.1002/cne.20842.
The distribution of putative nitric oxide synthase (NOS)-containing cells in the opisthobranch mollusc Aplysia californica was studied by using NADPH-diaphorase (NADPH-d) histochemistry in the CNS and peripheral organs. Chemosensory areas (the mouth area, rhinophores, and tentacles) express the most intense staining, primarily in the form of peripheral highly packed neuropil regions with a glomerular appearance as well as in epithelial sensory-like cells. These epithelial NADPH-d-reactive cells were small and had multiple apical ciliated processes exposed to the environment. NADPH-d processes were also found in the salivary glands, but there was no or very little staining in the buccal mass and foot musculature. In the CNS, most NADPH-d reactivity was associated with the neuropil of the cerebral ganglia, with the highest density of glomeruli-like NADPH-d-reactive neurites in the areas of the termini and around F and C clusters. A few NADPH-d-reactive neurons were also found in other central ganglia, including paired neurons in the buccal, pedal, and pleural ganglia and a few asymmetrical neurons in the abdominal ganglion. The distribution patterns of NADPH-d-reactive neurons did not overlap with other known neurotransmitter systems. The highly selective NADPH-d labeling revealed here suggests the presence of NOS in sensory areas both in the CNS and the peripheral organs of Aplysia and implies a role for NO as a modulator of chemosensory processing.
利用NADPH黄递酶（NADPH-d）组织化学方法，研究了后鳃亚纲软体动物加州海兔中枢神经系统（CNS）和外周器官中假定含一氧化氮合酶（NOS）细胞的分布情况。化学感受区域（口区、嗅角和触手）呈现出最强的染色，主要表现为外周高度密集的具有肾小球样外观的神经毡区域以及上皮感觉样细胞。这些上皮NADPH-d反应性细胞较小，有多个顶端纤毛突起暴露于外界环境。在唾液腺中也发现了NADPH-d反应过程，但在口腔团块和足部肌肉组织中没有或只有很少的染色。在中枢神经系统中，大多数NADPH-d反应性与脑神经节的神经毡相关，在终末区域以及F和C簇周围，肾小球样NADPH-d反应性神经突的密度最高。在其他中枢神经节中也发现了一些NADPH-d反应性神经元，包括颊神经节、足神经节和胸膜神经节中的成对神经元以及腹神经节中的一些不对称神经元。NADPH-d反应性神经元的分布模式与其他已知神经递质系统不重叠。此处揭示的高度选择性NADPH-d标记表明，在加州海兔的中枢神经系统和外周器官的感觉区域中存在NOS，并暗示NO作为化学感受处理调节剂的作用。
