一种用于检测6-酮-前列腺素F1α的灵敏放射免疫分析法：关于循环浓度的初步观察

A sensitive radioimmunoassay for 6-keto-prostaglandin F1alpha: preliminary observations on circulating concentrations.

作者信息

Mitchell M D

出版信息

Prostaglandins Med. 1978 Jul;1(1):13-21. doi: 10.1016/0161-4630(78)90072-1.

PMID:102000

Abstract

A radioimmunoassay for 6-keto-prostaglandin F1alpha (6-keto-PGF1alpha) is reported. The mean least detectable mass of 6-keto-PGF1alpha is 1.45 pg and the mean mass of added 6-keto-PGF1alpha required to displace zero-point binding by 50% is 94.2 pg. Cross-reactivities of the antiserum with all the prostaglandins and related compounds tested are less than 0.1% except for PGE2 and PGF2alpha which are respectively 0.16% and 0.80%. The assay is both accurate and reproducible and measured levels are in good agreement following extraction of 0.25--4.0 ml of plasma. Mean circulating concentrations of 6-keto-PGF1alpha in five non-pregnant humans, rhesus monkeys and sheep were 115, 65 and 25 pg/ml respectively.

摘要

本文报道了一种6-酮-前列腺素F1α（6-keto-PGF1α）的放射免疫分析方法。6-keto-PGF1α的平均最低检测质量为1.45皮克，使零点结合位移50%所需添加的6-keto-PGF1α的平均质量为94.2皮克。除PGE2和PGF2α的交叉反应率分别为0.16%和0.80%外，抗血清与所有测试的前列腺素及相关化合物的交叉反应率均低于0.1%。该分析方法准确且可重复，在提取0.25 - 4.0毫升血浆后，测量水平具有良好的一致性。五名非妊娠人类、恒河猴和绵羊的6-keto-PGF1α平均循环浓度分别为115、65和25皮克/毫升。