Morris R J, Potten C S
The Lankenau Medical Research Center, Wynnewood, Pennsylvania 19096, USA.
J Invest Dermatol. 1999 Apr;112(4):470-5. doi: 10.1046/j.1523-1747.1999.00537.x.
We have identified some unusually persistent label-retaining cells in the hair follicles of mice, and have investigated their role in hair growth. Three-dimensional reconstruction of dorsal underfur follicles from serial sections made 14 mo after complete labeling of epidermis and hair follicles in neonatal mice disclosed the presence of highly persistent label-retaining cells associated with the first-generation follicle involved in the production of the first wave of hairs, commonly called the bulge. The label-retaining cells were most often found on the ventral surface of the first-generation follicle, five cell positions from the base, near the attachment site of the arrector pilorum muscle. No label-retaining cells were found in the hair canal, sebaceous gland, or hair germ. These label-retaining cells remained in the follicle following induction of anagen by plucking of the hairs. Surprisingly, they were not part of the first wave of mitotic activity following plucking, but instead underwent mitosis beginning 42 h after plucking. Label-retaining cells or their labeled daughters were not found in the hair germs through 48 h following induction of anagen by plucking, but instead remained in their subsebaceous follicular location even upon completion of the hair growth cycle 21 d later. These label-retaining cells are, therefore, unlikely to contribute to the formation of a new anagen follicle.
我们在小鼠毛囊中鉴定出了一些异常持久的标记保留细胞，并研究了它们在毛发生长中的作用。对新生小鼠的表皮和毛囊进行完全标记14个月后，从连续切片中对背部绒毛毛囊进行三维重建，结果显示存在与参与第一波毛发产生的第一代毛囊相关的高度持久的标记保留细胞，通常称为隆突。标记保留细胞最常出现在第一代毛囊的腹侧表面，距基部五个细胞位置，靠近竖毛肌的附着部位。在毛干、皮脂腺或毛芽中未发现标记保留细胞。在拔毛诱导生长期后，这些标记保留细胞仍留在毛囊中。令人惊讶的是，它们不是拔毛后第一波有丝分裂活动的一部分，而是在拔毛后42小时开始进行有丝分裂。在拔毛诱导生长期后的48小时内，在毛芽中未发现标记保留细胞或其标记的子代细胞，而是即使在21天后毛发生长周期完成后，它们仍留在皮脂腺下的毛囊位置。因此，这些标记保留细胞不太可能有助于形成新的生长期毛囊。
