Gallet A, Angelats C, Erkner A, Charroux B, Fasano L, Kerridge S
Labororatoire de Génétique et Physiologie du Développement, UMR 9943 CNRS-Université, IBDM CNRS-INSERM-Université de la Méditerranée, Campus de Luminy Case 907, F-13288 Marseille, Cedex 09, France.
EMBO J. 1999 Apr 15;18(8):2208-17. doi: 10.1093/emboj/18.8.2208.
Wnt signalling is a key pathway for tissue patterning during animal development. In Drosophila, the Wnt protein Wingless acts to stabilize Armadillo inside cells where it binds to at least two DNA-binding factors which regulate specific target genes. One Armadillo-binding protein in Drosophila is the zinc finger protein Teashirt. Here we show that Wingless signalling promotes the phosphorylation and the nuclear accumulation of Teashirt. This process requires the binding of Teashirt to the C-terminal end of Armadillo. Finally, we present evidence that the serine/threonine kinase Shaggy is associated with Teashirt in a complex. We discuss these results with respect to current models of Armadillo/beta-catenin action for the transmission of the Wingless/Wnt pathway.
Wnt信号通路是动物发育过程中组织模式形成的关键途径。在果蝇中，Wnt蛋白无翅蛋白在细胞内发挥作用，使犰狳蛋白稳定，在细胞内它与至少两种调节特定靶基因的DNA结合因子结合。果蝇中的一种犰狳结合蛋白是锌指蛋白无尾。在这里我们表明，无翅信号通路促进无尾的磷酸化和核积累。这个过程需要无尾与犰狳蛋白的C末端结合。最后，我们提供证据表明丝氨酸/苏氨酸激酶蓬乱与无尾在一个复合物中相关联。我们根据目前关于犰狳蛋白/β-连环蛋白在无翅/Wnt信号通路传递中的作用模型来讨论这些结果。
