Owen Jared H, Wagner Daniel E, Chen Chun-Chieh, Petersen Christian P, Reddien Peter W
Howard Hughes Medical Institute, MIT Biology and Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA.
Howard Hughes Medical Institute, MIT Biology and Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA Department of Molecular Biosciences, Northwestern University, 2205 Tech Drive, Hogan Hall Room 2-144, Evanston, IL 60208, USA.
Development. 2015 Mar 15;142(6):1062-72. doi: 10.1242/dev.119685. Epub 2015 Feb 27.
Regeneration requires that the identities of new cells are properly specified to replace missing tissues. The Wnt signaling pathway serves a central role in specifying posterior cell fates during planarian regeneration. We identified a gene encoding a homolog of the Teashirt family of zinc-finger proteins in the planarian Schmidtea mediterranea to be a target of Wnt signaling in intact animals and at posterior-facing wounds. Inhibition of Smed-teashirt (teashirt) by RNA interference (RNAi) resulted in the regeneration of heads in place of tails, a phenotype previously observed with RNAi of the Wnt pathway genes β-catenin-1, wnt1, Dvl-1/2 or wntless. teashirt was required for β-catenin-1-dependent activation of posterior genes during regeneration. These findings identify teashirt as a transcriptional target of Wnt signaling required for Wnt-mediated specification of posterior blastemas.
组织再生需要正确指定新细胞的身份以替代缺失的组织。Wnt信号通路在涡虫再生过程中确定后端细胞命运方面起着核心作用。我们在真涡虫中鉴定出一个编码锌指蛋白茶衫家族同源物的基因，该基因在完整动物和后端伤口处是Wnt信号的靶标。通过RNA干扰（RNAi）抑制真涡虫茶衫基因（teashirt）会导致头部再生而非尾部，这种表型先前在Wnt通路基因β-连环蛋白-1、wnt1、Dvl-1/2或wntless的RNAi实验中观察到。在再生过程中，teashirt是β-连环蛋白-1依赖的后端基因激活所必需的。这些发现确定teashirt是Wnt介导的后端芽基特化所需的Wnt信号的转录靶标。
