Willershausen B, Schäfer D, Pistorius A, Schulze R, Mann W
Department of Restorative Dentistry and Periodontology, Augustusplatz 2, D-55131 Mainz, Germany.
Eur J Med Res. 1999 Apr 27;4(4):149-55.
Tooth-colored resin-based restoration materials have gained enormous significance in recent decades. Since the composites presently available still evidence various weaknesses, intensive research continues concerning them. In this study we tested the biocompatibility of six resin-based materials (Charisma, Solitaire, Definite, Tetric ceram, Ariston pHc and Compoglass) by means of cultured human gingival fibroblasts. Over a period of 9 days the cured resin-based materials were incubated with the fibroblasts. Cell growth, cell viability, and protein content was measured. The release of prostaglandin (PGE subset2) was also determined. There was a reduction of cell functions, related to the different resin-based materials. Protein content of the fibroblasts was significantly reduced (Kruskal Wallis test; p = 0.0001), depending on the material tested. Definite induced only minimal irritation, and there was correspondingly little contrast to the protein content of the controls. Solitaire induced the highest levels of irritation and the greatest reduction in protein content. Lactate production of fibroblasts also showed some significant reduction in contact with particular materials (p = 0.005). There were only small differences among the tested materials as concerns measured PGE subset2 release of the fibroblasts. The data suggest that the methods here employed may be quite useful in testing the cytotoxic potential of newly developed resin-based materials
近几十年来，树脂基牙齿修复材料变得极为重要。由于目前可用的复合材料仍存在各种弱点，因此对它们的深入研究仍在继续。在本研究中，我们通过培养的人牙龈成纤维细胞测试了六种树脂基材料（卡瑞斯玛、纸牌、定形、泰克西姆陶瓷、阿瑞斯顿pHc和复合玻璃）的生物相容性。在9天的时间里，将固化的树脂基材料与成纤维细胞一起培养。测量细胞生长、细胞活力和蛋白质含量。还测定了前列腺素（PGE2亚型）的释放。与不同的树脂基材料相关的细胞功能有所下降。根据所测试的材料，成纤维细胞的蛋白质含量显著降低（Kruskal Wallis检验；p = 0.0001）。定形材料仅引起最小程度的刺激，与对照的蛋白质含量相应地几乎没有差异。纸牌材料引起的刺激水平最高，蛋白质含量下降最大。成纤维细胞的乳酸产生在与特定材料接触时也显示出一些显著降低（p = 0.005）。在所测试的材料中，就成纤维细胞的PGE2亚型释放量而言，差异很小。数据表明，这里采用的方法在测试新开发的树脂基材料的细胞毒性潜力方面可能非常有用。
