基于树脂的牙科修复材料对WEHI 13 var成纤维细胞细胞毒性的体外评估。
In vitro assessment of cytotoxicity of resin-based dental restorative materials on WEHI 13 var fibroblasts.
作者信息
Bakopoulou Athina A, Triviai Ioanna N, Tsiftsoglou Asterios S, Garefis Paylos D
机构信息
Department of Fixed & Implant Prosthodontics, School of Dentistry, Aristotle University of Thessaloniki, Greece.
出版信息
Int J Prosthodont. 2006 Jan-Feb;19(1):13-6.
PURPOSE
The aim of this study was to assess the cytotoxicity of 4 resin composites' eluates on WEHI 13 Var fibroblasts as they aged in a biologic medium.
MATERIALS AND METHODS
Cytotoxicity was determined by counting the number of viable cells by trypan blue exclusion. Morphologic changes attributable to cytotoxicity were observed by May-Grunwald-Giemsa cytologic staining and microscopic examination. DNA gel electrophoresis was performed to detect possible genotoxicity and DNA damage.
RESULTS
All resin composite eluates, except that for Targis, caused a pronounced cytotoxicity during the first 72 hours that gradually decreased after 2 weeks of aging. Severe morphologic alterations and pronounced DNA damage were also observed.
CONCLUSION
These findings indicate that resin-based dental restorative materials release agents cytotoxic and genotoxic to fibroblasts. Cytotoxicity is gradually decreased as the composite resins age in a biologic-relevant medium.
目的
本研究的目的是评估4种树脂复合材料洗脱液在生物培养基中老化时对WEHI 13 Var成纤维细胞的细胞毒性。
材料与方法
通过台盼蓝排斥法计数活细胞数量来确定细胞毒性。通过May-Grunwald-Giemsa细胞染色和显微镜检查观察细胞毒性引起的形态学变化。进行DNA凝胶电泳以检测可能的遗传毒性和DNA损伤。
结果
除Targis的洗脱液外，所有树脂复合材料洗脱液在最初72小时内均引起明显的细胞毒性，老化2周后逐渐降低。还观察到严重的形态学改变和明显的DNA损伤。
结论
这些发现表明，树脂基牙科修复材料释放对成纤维细胞具有细胞毒性和遗传毒性的物质。随着复合树脂在生物相关培养基中老化，细胞毒性逐渐降低。
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