Labban Nawaf, Song Fengyu, Al-Shibani Nouf, Windsor L Jack
Department of Restorative Dentistry-Prosthodontics, Indiana University School of Dentistry, Indianapolis, Indiana 46202, USA.
J Prosthet Dent. 2008 Nov;100(5):390-7. doi: 10.1016/S0022-3913(08)60242-5.
Several studies have reported that polymerized resin materials may release agents into surrounding tissues. These agents could alter cytokine/growth factor expression.
The purpose of this study was to determine the effects that provisional acrylic resins have on cell toxicity and the expression of cytokines/growth factors from human gingival fibroblasts (HGFs).
The materials used in this study were chemically activated bis-acryl composite (Chem-Bis), chemically activated polyethyl methacrylate (Chem-PEMA), chemically activated polymethyl methacrylate (Chem-PMMA), and heat-activated polymethyl methacrylate (Heat-PMMA) resins. HGFs were incubated for 72 hours in the presence of eluate from each resin and in the absence of any eluate (negative control). The conditioned media were then collected and stored at -70 degrees C. Cell toxicity was determined using a lactate dehydrogenase method. Cytokine/growth factor expression was examined using cytokine antibody arrays. The experiments were repeated 3 times. The data were analyzed with 1-way ANOVA, Mann-Whitney test, and 1-sample t test (alpha=.05).
There was no significant cell toxicity observed from the eluates. The cytokine/growth factor expression induced by Chem-Bis was significantly greater than the control for growth-regulated oncogene (GRO) (P<.001), monocyte chemoattractant protein-1 (MCP-1) (P=.031), and tumor necrosis factor-beta (TNF)-beta (P=.009). For Chem-PEMA, the cytokine/growth factor expression was significantly greater than the control for GRO-alpha (P=.022), interleukin (IL)-13 (P=.031), and TNF-alpha (P=.017). The cytokines/growth factors induced by Chem-PEMA were significantly less than the control (P=.008) and Chem-Bis for IL-8 (P=.042). The expression induced by Chem-PMMA was significantly greater than the control for IL-13 (P=.036), IL-1 alpha (P=.003), IL-2 (P=.020), and IL-5 (P=.045). Finally, Heat-PMMA induced significantly greater levels than the control for GRO (P<.001) and IL-13 (P=.008).
This study demonstrated that the resins evaluated were nontoxic to the HGFs. There were changes in the cytokine/growth factor levels that were statistically significant, but may not be clinically significant.
多项研究报告称，聚合树脂材料可能会向周围组织释放物质。这些物质可能会改变细胞因子/生长因子的表达。
本研究的目的是确定临时丙烯酸树脂对人牙龈成纤维细胞（HGFs）的细胞毒性以及细胞因子/生长因子表达的影响。
本研究使用的材料为化学活化双丙烯酸酯复合材料（Chem-Bis）、化学活化聚甲基丙烯酸乙酯（Chem-PEMA）、化学活化聚甲基丙烯酸甲酯（Chem-PMMA）和热活化聚甲基丙烯酸甲酯（Heat-PMMA）树脂。将HGFs在每种树脂的洗脱液存在下孵育72小时，并在无任何洗脱液的情况下孵育（阴性对照）。然后收集条件培养基并储存在-70℃。使用乳酸脱氢酶法测定细胞毒性。使用细胞因子抗体阵列检测细胞因子/生长因子的表达。实验重复3次。数据采用单向方差分析、曼-惠特尼检验和单样本t检验进行分析（α = 0.05）。
洗脱液未观察到明显的细胞毒性。Chem-Bis诱导的细胞因子/生长因子表达在生长调节致癌基因（GRO）（P < 0.001）、单核细胞趋化蛋白-1（MCP-1）（P = 0.031）和肿瘤坏死因子-β（TNF）-β（P = 0.009）方面显著高于对照组。对于Chem-PEMA，细胞因子/生长因子表达在GRO-α（P = 0.022）、白细胞介素（IL）-13（P = 0.031）和TNF-α（P = 0.017）方面显著高于对照组。Chem-PEMA诱导的细胞因子/生长因子在IL-8方面显著低于对照组（P = 0.008）和Chem-Bis（P = 0.042）。Chem-PMMA诱导的表达在IL-13（P = 0.036）、IL-1α（P = 0.003）、IL-2（P = 0.020）和IL-5（P = 0.045）方面显著高于对照组。最后，Heat-PMMA诱导的水平在GRO（P < 0.001）和IL-13（P = 0.008）方面显著高于对照组。
本研究表明，所评估的树脂对HGFs无毒。细胞因子/生长因子水平存在统计学上的显著变化，但可能无临床意义。
