Bacelar Polyanna A A, Monteiro Kerla J L, Dos Santos Jéssica P, Silva Denilson de A E, Leal Daniella N, Almeida Mayron M, Evangelista Brenda B C, de Oliveira-Neto Francisco M, Carvalho-Costa Filipe A
Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
Regional Office Oswaldo Cruz Foundation-Piauí, Teresina, Brazil.
J Trop Med. 2022 Aug 23;2022:7251922. doi: 10.1155/2022/7251922. eCollection 2022.
Some helminth species belonging to the order Strongylida are parasites of the digestive tract of vertebrates, including man and domestic animals. In humans, infections with spp. and spp. may be misdiagnosed as hookworm disease on parasitological stool examination, mainly in regions where these infections are coendemic, since eggs released in hosts' feces are morphologically similar. This study presents the morphologic characterization of Strongylida larvae recovered from humans and pigs living in close proximity, exploring putative zoonotic cycles. One hundred three humans and 27 pigs were included in low-resource rural communities in the state of Piauí, northeastern Brazil. Strongylida eggs were present in 12 (11.7%) humans and 23 (85.2%) pigs through conventional parasitological examination. Strongylida-positive fecal samples were submitted to coprocultures using the Harada-Mori technique. All 22 larvae obtained from human feces were classified as hookworms (Ancylostomatidae). From a total of 37 larvae obtained from swine, 23 (62.3%) were classified as , 6 (16.2%) were , and 4 (10.8%) were . Four larvae (10.8%) obtained from pigs were classified as . The morphological study of filariform larvae obtained in coprocultures is a useful and inexpensive tool in the screening of intestinal helminthiasis in a One Health approach.
一些圆线目蠕虫是脊椎动物（包括人类和家畜）消化道的寄生虫。在人类中，感染 spp. 和 spp. 在粪便寄生虫学检查中可能被误诊为钩虫病，主要是在这些感染共同流行的地区，因为宿主粪便中排出的虫卵在形态上相似。本研究呈现了从生活在相近区域的人类和猪体内回收的圆线目幼虫的形态学特征，探索可能的人畜共患病循环。在巴西东北部皮奥伊州的资源匮乏农村社区纳入了103名人类和27头猪。通过传统寄生虫学检查，12名（11.7%）人类和23头（85.2%）猪体内存在圆线目虫卵。对圆线目阳性粪便样本采用原田-森氏技术进行粪便培养。从人类粪便中获得的所有22条幼虫均被归类为钩虫（钩口科）。从猪粪便中获得的37条幼虫中，23条（62.3%）被归类为 ，6条（16.2%）被归类为 ，4条（10.8%）被归类为 。从猪体内获得的4条幼虫（10.8%）被归类为 。在粪便培养中获得的丝状幼虫的形态学研究是以“同一健康”方法筛查肠道蠕虫病的一种有用且廉价的工具。
