Modifying secretion and post-translational processing in insect cells.
作者信息
Ailor E, Betenbaugh M J
机构信息
Department of Chemical Engineering, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA.
出版信息
Curr Opin Biotechnol. 1999 Apr;10(2):142-5. doi: 10.1016/s0958-1669(99)80024-x.
Abstract
The baculovirus-insect cell system is a valuable tool for the expression of heterologous proteins. Due to limitations in the intracellular processing environment, however, heterologous secreted and membrane proteins are often insoluble, poorly processed, or contain 'non-human' modifications. Recent attempts to modify the insect cell secretory pathway by overexpressing processing factors have demonstrated the potential to overcome these limitations.
摘要
Zotero 插件