Bartman A E, Buisine M P, Aubert J P, Niehans G A, Toribara N W, Kim Y S, Kelly E J, Crabtree J E, Ho S B
Department of Medicine, University of Minnesota, Minneapolis, USA.
J Pathol. 1998 Dec;186(4):398-405. doi: 10.1002/(SICI)1096-9896(199812)186:4<398::AID-PATH192>3.0.CO;2-X.
Secretory mucins play an important role in the cytoprotection of epithelial surfaces and are used as tumour markers in a variety of cancers. The MUC6 secretory mucin was originally isolated from a gastric cDNA library. The aim was to determine the specific type and location of MUC6 mucin gene expression in a wide range of human adult and fetal epithelial tissues. In situ hybridization, RNA analysis, and immunohistochemistry were used to quantify and localize mucin gene expression. The data obtained show that MUC6 is highly expressed in gastric mucosa, duodenal Brunner's glands, gall bladder, seminal vesicle, pancreatic centroacinar cells and ducts, and periductal glands of the common bile duct; focal expression is seen in basal endometrial and endocervical glands. MUC6 epitopes were also highly expressed in 7/10 pancreatic cancers and 7/10 cholangiocarcinomas and focally expressed in 4/10 endocervical adenocarcinomas. Expression of MUC6 occurs early in fetal development and was observed in Brunner's glands and pancreatic ducts at 18-19 weeks and in gastric glands at 20 weeks' gestation. The tissue distribution of the MUC6 secretory mucin indicates that it may function to protect epithelial tissues from a wide range of substances. Expression of MUC6 is frequently preserved in pancreatic and bile duct adenocarcinomas, but it is only sparsely expressed in endocervical carcinomas.
分泌性黏蛋白在上皮表面的细胞保护中发挥重要作用，并在多种癌症中用作肿瘤标志物。MUC6分泌性黏蛋白最初是从胃cDNA文库中分离出来的。目的是确定MUC6黏蛋白基因在广泛的成人和胎儿上皮组织中的具体表达类型和位置。采用原位杂交、RNA分析和免疫组织化学方法对黏蛋白基因表达进行定量和定位。所得数据表明，MUC6在胃黏膜、十二指肠布伦纳腺、胆囊、精囊、胰腺中央腺泡细胞和导管以及胆总管的导管周围腺体中高度表达；在子宫内膜和宫颈管腺体基底有局灶性表达。MUC6表位在7/10的胰腺癌和7/10的胆管癌中也高度表达，在4/10的宫颈管腺癌中有局灶性表达。MUC6的表达在胎儿发育早期就已出现，在妊娠18 - 19周时在布伦纳腺和胰腺导管中观察到，在妊娠20周时在胃腺中观察到。MUC6分泌性黏蛋白的组织分布表明它可能起到保护上皮组织免受多种物质侵害的作用。MUC6的表达在胰腺和胆管腺癌中常常得以保留，但在宫颈管癌中仅呈稀疏表达。
