Günel E, Cağlayan F, Cağlayan O, Akillioğlu I
Department of Pediatric Surgery, Medical School of Selçuk University, Konya, Turkey.
J Pediatr Surg. 1999 Mar;34(3):405-7. doi: 10.1016/s0022-3468(99)90486-4.
This study was designed to determine the tissue levels of reactive oxygen radicals in caustic esophageal burns in a rat model.
Forty rats were divided into four groups of 10 animals each. The control rats were uninjured in group A, and the others were injured rats in groups B, C, and D. Through a median laparatomy incision, a 1.5-cm abdominal esophageal segment was isolated and tied with 2-0 chromic sutures in all groups as described by Gehanno. One milliliter of 10% sodium hydroxide solution in groups B, C, and D and 0.9% saline solution in group A were instilled through the isolated segment via a no. 24 cannula for 3 minutes, then the esophagus was rinsed for 1 minute with distilled water. The studied 1.5 cm of the abdominal esophagus was removed from each animal 24 hours after caustic injury in group B, 48 hours after in group C, and 72 hours after in group D. In group A, studied uninjured segments were removed for biochemical investigation. Tissue malondialdehyde (MDA) and glutathione (GSH) levels were determined for each group.
The mean MDA levels in esophageal tissue were significantly higher in groups B, C, and D than in group A (P < .05). Moreover, the mean GSH levels in the same esophageal tissues were significantly lower in groups C and D than in groups A and B (P < .05).
The reactive oxygen radicals may be play an important role in early phase of caustic esophageal burns by increasing the tissue damage.
本研究旨在确定大鼠模型中腐蚀性食管烧伤时活性氧自由基的组织水平。
40只大鼠分为四组，每组10只。A组为未受伤的对照大鼠，B、C、D组为受伤大鼠。如Gehanno所述，所有组均通过正中剖腹切口分离出1.5厘米的腹部食管段，并用2-0铬制缝线结扎。B、C、D组通过24号套管经分离段注入1毫升10%氢氧化钠溶液3分钟，然后用蒸馏水冲洗食管1分钟。B组在腐蚀性损伤后24小时、C组在48小时、D组在72小时从每只动物身上取出研究的1.5厘米腹部食管。A组取出未受伤的研究段进行生化研究。测定每组组织丙二醛（MDA）和谷胱甘肽（GSH）水平。
B、C、D组食管组织中MDA平均水平显著高于A组（P < .05）。此外，C组和D组相同食管组织中GSH平均水平显著低于A组和B组（P < .05）。
活性氧自由基可能通过增加组织损伤在腐蚀性食管烧伤早期起重要作用。
