Okata Yuichi, Hisamatsu Chieko, Hasegawa Tomomi, Nishijima Eiji, Okita Yutaka
Division of Pediatric Surgery, Department of Surgery, Kobe University Graduate School of Medicine, Kobe, 650-0017, Japan.
Pediatr Surg Int. 2011 Jan;27(1):73-80. doi: 10.1007/s00383-010-2711-5.
To investigate the optimal concentration of sodium hydroxide (NaOH) on esophageal stricture formation in rats to establish an animal model of benign esophageal stricture (BES).
Corrosive esophageal burn was produced by internal application of different concentrations of NaOH to the distal esophagus in rats. As much as 66 male rats were randomly divided into eight groups: Group A (control, n = 6), Group B (sham-operated group, n = 6), Group C (5% NaOH, n = 8), Group D (10% NaOH, n = 8), Group E (20% NaOH, n = 8), Group F (30% NaOH, n = 10), Group G (40% NaOH, n = 14), and Group H (50% NaOH, n = 6). Surviving rats were killed at 28 days. The survival rate, body weight gain, symptoms, and histopathological changes were assessed.
The mortality rate was high in Groups G and H (73 and 67%). The prevalence of symptoms of BES was 43% in Groups D and E, 50% in Group F, 75% in Group G, and 100% in Group H. Statistically significant stricture formation of the esophagus was observed in Groups F and G. The degree of tissue damage was significantly higher in Groups E, F, and G.
A high concentration of NaOH of 30% was required to establish a survivable BES model in rats.
研究氢氧化钠（NaOH）的最佳浓度对大鼠食管狭窄形成的影响，以建立良性食管狭窄（BES）动物模型。
通过向大鼠食管远端内注入不同浓度的NaOH造成腐蚀性食管烧伤。66只雄性大鼠随机分为八组：A组（对照组，n = 6）、B组（假手术组，n = 6）、C组（5% NaOH，n = 8）、D组（10% NaOH，n = 8）、E组（20% NaOH，n = 8）、F组（30% NaOH，n = 10）、G组（40% NaOH，n = 14）和H组（50% NaOH，n = 6）。存活大鼠于28天时处死，评估存活率、体重增加、症状及组织病理学变化。
G组和H组死亡率较高（分别为73%和67%）。D组和E组BES症状发生率为43%，F组为50%，G组为75%，H组为100%。F组和G组观察到食管有统计学意义的狭窄形成。E组、F组和G组组织损伤程度明显更高。
需要30%的高浓度NaOH才能在大鼠中建立可存活的BES模型。
