Lasobras J, Dellunde J, Jofre J, Lucena F
Departament de Microbiologia, Universitat de Barcelona, Spain.
J Appl Microbiol. 1999 Apr;86(4):723-9. doi: 10.1046/j.1365-2672.1999.00722.x.
A method based on the treatment of sludge with beef extract recovered, with similar efficiency, the three groups of bacteriophages studied from different kinds of sludges. The three groups of bacteriophages were found in high numbers in the different sludge types, the highest value being that of somatic coliphages in primary sludge of a biological treatment plant (1.1 x 10(5) pfu g-1) and the lowest being that of Bacteroides fragilis phages (110 pfu g-1) in de-watered, anaerobically, mesophilically-digested sludge. All phages studied accumulated in the sludges. In primary and activated sludges, all three types accumulated similarly but in lime-treated sludge and de-watered, anaerobically, mesophilically-digested sludge, the relative proportion of F-specific bacteriophages decreased significantly with respect to somatic coliphages and bacteriophages infecting B. fragilis. All phages survived successfully in stored sludge, depending on the temperature, and again, F-specific bacteriophages survived less successfully than the others.
一种基于用牛肉膏处理污泥的方法，以相似的效率从不同类型的污泥中回收了所研究的三组噬菌体。在不同类型的污泥中发现了大量的这三组噬菌体，其中生物处理厂初级污泥中体细胞噬菌体的数量最高（1.1×10⁵ 噬菌斑形成单位/克），而在厌氧中温消化的脱水污泥中脆弱拟杆菌噬菌体的数量最低（110 噬菌斑形成单位/克）。所有研究的噬菌体都在污泥中积累。在初级污泥和活性污泥中，三种类型的噬菌体积累情况相似，但在石灰处理的污泥以及厌氧中温消化的脱水污泥中，F 特异性噬菌体相对于体细胞噬菌体和感染脆弱拟杆菌的噬菌体的相对比例显著降低。所有噬菌体在储存的污泥中都能成功存活，这取决于温度，而且 F 特异性噬菌体的存活情况再次不如其他噬菌体。
