Jalava K, Hielm S, Hirvi U, Hänninen M L
Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, University of Helsinki, Finland.
Lett Appl Microbiol. 1999 Apr;28(4):269-74. doi: 10.1046/j.1365-2672.1999.00527.x.
A scheme for the rapid identification of Helicobacter spp. using restriction fragment length polymorphism digestion profiles of PCR amplified 23S rRNA genes is described. The efficacy of this scheme for speciation of the closely related gastric species H. felis, H. bizzozeronii and H. salomonis was evaluated. It was difficult to distinguish between some RFLP profiles obtained and often, more than one profile was seen with each species examined. Some evidence was found that the 23S rRNA gene copies of these species may not be identical. Moreover, the identification scheme was ineffective in discriminating these species from each other, although they could be differentiated, as a group, from other Helicobacter spp. The results indicate that this scheme should be carefully evaluated with a number of isolates if it is to be applied to additional, highly related Helicobacter spp.
本文描述了一种利用聚合酶链反应（PCR）扩增的23S rRNA基因的限制性片段长度多态性消化图谱快速鉴定幽门螺杆菌属（Helicobacter spp.）的方法。评估了该方法对密切相关的胃部菌种——猫幽门螺杆菌（H. felis）、比氏幽门螺杆菌（H. bizzozeronii）和所罗门幽门螺杆菌（H. salomonis）进行分类的有效性。所获得的一些限制性片段长度多态性（RFLP）图谱难以区分，而且在所检测的每个菌种中常常能看到不止一种图谱。有证据表明，这些菌种的23S rRNA基因拷贝可能并不相同。此外，尽管这些菌种作为一个整体可以与其他幽门螺杆菌属菌种区分开来，但该鉴定方法在区分它们彼此之间的差异时效果不佳。结果表明，如果要将该方法应用于其他高度相关的幽门螺杆菌属菌种，需要用多个分离株对其进行仔细评估。
